Precision Red Advanced Protein Assay: 1x stock

Precision Red Advanced Protein Assay: 1x stock

Product uses include measuring protein in

  • Cell extracts
  • Serum samples
  • Medium to high protein concentration solutions
  • Protein requiring GLP and higher standards
  • Purified proteins and antibodies

The Precision Red Advanced Protein Assay Reagent is designed to optimize the speed and accuracy of protein measurement. The reagent combines the useful properties of low protein to protein variance (See Fig 1) an
 d a wide protein concentration for a robust assay. The assay can measure accurately protein concentrations ranging from 0.25 to 50 mg/ml and is detergent compatible. A simple one step procedure results in a red to blue color change within 1 min, which can be recognized by measuring absorbance at 600 nm.

This reagent is so reproducible with a wide range of proteins and protein concentrations that it is employed to accurately determine the concentration in all of Cytoskeleton, Inc's protein products.


Figure 1: The low protein to protein variance with ADV01 and ADV02. A comparison of ADV01, ADV02, Bradford and Lowry assays on five different proteins.

The Precision Red Advanced Protein Assay makes it very easy and quick to measure prottein concentrations. A general method looks as follows:

  1. Mix protein solution with ADV02 reagent (e.g. 10 µl sample with 1.0 ml ADV02)
  2. Incubate 1 min
  3. Read absorbance at 600 nm

Detergent and chemical compatibility
The Precision Red Advanced Protein Assay is a highly stable assay and it tolerates a wide range of reagents. See the Advanced Protein Assay reagent compatibility page for a full list of reagents that have been tested.

Using the Precision Red Advanced Protein Assay in cell extracts
Since the Precision Red Advanced Protein Assay is highly compatible with non-ionic detergents (see the ADV Chemical tolerance page), this reagent is ideal for measuring protein concentrations in cell and tissue extracts. Furthermore, since protein concentration can be measured after only 1 min of incubation with the reagent, it allows the user to proceed with the experiment quicker than any other protein assay, something that can be of big importance when working with labile cell extracts.

Cytoskeleton, Inc. recommends using ADV02 for all our kits and assays that involve cell or tissue lysis. In the G-LISA™ small G-protein activation kits, we have even included this reagent to ensure that the user gets the most accurate and quickest possible measurement of the protein concentration in the cell lysates generated.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at

AuthorTitleJournalYearArticle Link
Cruz-Nova, Pedro et al.131I-C19 Iodide Radioisotope and Synthetic I-C19 Compounds as K-Ras4B–PDE6δ Inhibitors: A Novel Approach against Colorectal Cancer—Biological Characterization, Biokinetics and DosimetryMolecules 2022, Vol. 27, Page 54462022ISSN 1420--3049
Hassin, Ori et al.Different hotspot p53 mutants exert distinct phenotypes and predict outcome of colorectal cancer patientsNature Communications 2022 13:12022ISSN 2041--1723
Xu, Weiyi et al.Paxillin promotes breast tumor collective cell invasion through maintenance of adherens junction integrityMolecular Biology of the Cell2022ISSN 1939-4586
Xu, Weiyi et al.Paxillin promotes breast tumor collective cell invasion through maintenance of adherens junction integrityMolecular biology of the cell2022ISSN 1939-4586
Shields, Mario A. et al.Gα13 loss in Kras/Tp53 mouse model of pancreatic tumorigenesis promotes tumors susceptible to rapamycinCell Reports2022ISSN 2211-1247
Datta, Gaurav et al.Endolysosome Localization of ERα Is Involved in the Protective Effect of 17α-Estradiol against HIV-1 gp120-Induced Neuronal InjuryThe Journal of neuroscience : the official journal of the Society for Neuroscience2021ISSN 1529-2401
Hryniewicz-Jankowska, Anita et al.Article the effect of neddylation inhibition on inflammation-induced mmp9 gene expression in esophageal squamous cell carcinomaInternational Journal of Molecular Sciences2021ISSN 1422-0067
Le, Anh Hoang et al.Cyri-a limits invasive migration through macropinosome formation and integrin uptake regulationJournal of Cell Biology2021ISSN 1540-8140
Nehme, Jamil et al.High dietary protein and fat contents exacerbate hepatic senescence and SASP in miceFEBS Journal2021ISSN 1742-4658
Liao, Liang et al.Disrupting RhoA activity by blocking Arhgef3 expression mitigates microglia-induced neuroinflammation post spinal cord contusionJournal of Neuroimmunology2021ISSN 1872-8421
Dolmatova, Elena V et al.Endothelial Poldip2 regulates sepsis-induced lung injury via Rho pathway activationCardiovascular Research2021ISSN 0008--6363
Rojas-Colón, Luis A. et al.4R-cembranoid confers neuroprotection against LPS-induced hippocampal inflammation in miceJournal of Neuroinflammation2021ISSN 1742-2094
Bordeleau, Francois et al.Tissue transglutaminase 2 regulates tumor cell tensional homeostasis by increasing contractilityJournal of Cell Science2020ISSN 1477-9137
Papalazarou, Vassilis et al.The creatine–phosphagen system is mechanoresponsive in pancreatic adenocarcinoma and fuels invasion and metastasisNature Metabolism2020ISSN 2522-5812
Dias Gomes, Martim et al.Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrityNature Communications2019ISSN 2041-1723
Stephens, Matthew et al.Mesenteric lymphatic alterations observed during DSS induced intestinal inflammation are driven in a TLR4-PAMP/DAMP discriminative mannerFrontiers in Immunology2019ISSN 1664-3224
Cederquist, Gustav Y. et al.Specification of positional identity in forebrain organoidsNature Biotechnology2019ISSN 1546-1696
Tedeschi, Andrea et al.ADF/Cofilin-Mediated Actin Turnover Promotes Axon Regeneration in the Adult CNSNeuron2019ISSN 1097-4199
Kikuchi, Daniel S. et al.Poldip2 mediates blood-brain barrier disruption in a model of sepsis-associated encephalopathyJournal of Neuroinflammation2019ISSN 1742-2094
Umesalma, Shaikamjad et al.RABL6A inhibits tumor-suppressive PP2A/AKT signaling to drive pancreatic neuroendocrine tumor growthJournal of Clinical Investigation2019ISSN 1558-8238
Paonessa, Francesco et al.Microtubules Deform the Nuclear Membrane and Disrupt Nucleocytoplasmic Transport in Tau-Mediated Frontotemporal DementiaCell Reports2019ISSN 2211-1247
Juin, Amelie et al.N-WASP Control of LPAR1 Trafficking Establishes Response to Self-Generated LPA Gradients to Promote Pancreatic Cancer Cell MetastasisDevelopmental Cell2019ISSN 1878-1551
Cruz-Nova, Pedro et al.The small organic molecule C19 binds and strengthens the KRAS4b-PDEδ complex and inhibits growth of colorectal cancer cells in vitro and in vivo 11 Medical and Health Sciences 1112 Oncology and CarcinogenesisBMC Cancer2018ISSN 1471-2407
Casique-Aguirre, Diana et al.KRas4B-PDE6δ complex stabilization by small molecules obtained by virtual screening affects Ras signaling in pancreatic cancer 06 Biological Sciences 0601 Biochemistry and Cell BiologyBMC Cancer2018ISSN 1471-2407
McQueeney, Kelley E. et al.Targeting ovarian cancer and endothelium with an allosteric PTP4A3 phosphatase inhibitorOncotarget2018ISSN 1949-2553
Zhu, Yili et al.An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated ProteinBio-Protocol2018ISSN 2331--8325
Proto, Jonathan D. et al.Regulatory T Cells Promote Macrophage Efferocytosis during Inflammation ResolutionImmunity2018ISSN 1097-4180
Mangiatordi, Giuseppe Felice et al.Automated identification of structurally heterogeneous and patentable antiproliferative hits as potential tubulin inhibitorsChemical Biology and Drug Design2018ISSN 1747-0285
Meiring, Joyce C.M. et al.Co-polymers of Actin and Tropomyosin Account for a Major Fraction of the Human Actin CytoskeletonCurrent Biology2018ISSN 0960-9822
Alhasani, Reem Hasaballah et al.Gypenosides protect retinal pigment epithelium cells from oxidative stressFood and Chemical Toxicology2018ISSN 1873-6351
Patnaik, Sarita Rani et al.RPGR protein complex regulates proteasome activity and mediates store-operated calcium entryOncotarget2018ISSN 1949-2553
Borin, Mirta et al.Rac1 activation links tau hyperphosphorylation and Aß dysmetabolism in Alzheimer's diseaseActa Neuropathologica Communications2018ISSN 2051-5960
Zhang, Xiaoqing et al.Alterations of MEK1/2-ERK1/2, IFNγ and Smad2/3 associated Signalling pathways during cryopreservation of ASCs affect their differentiation towards VSMC-like cellsStem Cell Research2018ISSN 1876-7753
Horita, Henrick et al.Utilizing a comprehensive immunoprecipitation enrichment system to identify an endogenous post-translational modification profile for target proteinsJournal of Visualized Experiments2018ISSN 1940-087X
Hendricks, Louie et al.Milk fat globule-epidermal growth factor-factor VIII–derived peptide MSP68 is a cytoskeletal immunomodulator of neutrophils that inhibits Rac1Journal of Surgical Research2017ISSN 1095-8673
Huang, Jia Lin et al.Lipoprotein-biomimetic nanostructure enables efficient targeting delivery of siRNA to Ras-activated glioblastoma cells via macropinocytosisNature Communications2017ISSN 2041-1723
Albalawi, Aishah et al.Protective effect of carnosic acid against acrylamide-induced toxicity in RPE cellsFood and Chemical Toxicology2017ISSN 1873-6351
Mangiatordi, Giuseppe Felice et al.Novel chemotypes targeting tubulin at the colchicine binding site and unbiasing P-glycoproteinEuropean Journal of Medicinal Chemistry2017ISSN 1768-3254
Chen, Lihua et al.Smooth muscle-alpha actin inhibits vascular smooth muscle cell proliferation and migration by inhibiting rac1 activityPLoS ONE2016ISSN 1932-6203
Zan, G. Y. et al.p38 mitogen-activated protein kinase activation in amygdala mediates κ opioid receptor agonist U50,488H-induced conditioned place aversionNeuroscience2016ISSN 1873-7544
Lampi, Marsha C. et al.Simvastatin ameliorates matrix stiffness-mediated endothelial monolayer disruptionPLoS ONE2016ISSN 1932-6203
Chen, Weihua et al.High RhoA expression at the tumor front in clinically localized prostate cancer and association with poor tumor differentiationOncology Letters2016ISSN 1792-1082
Castillo-Pichardo, Linette et al.Dietary grape polyphenol resveratrol increases mammary tumor growth and metastasis in immunocompromised mice2013PMID 23298290
Zilberter, Misha et al.Dietary energy substrates reverse early neuronal hyperactivity in a mouse model of Alzheimer's diseaseJournal of Neurochemistry2013ISSN 1471--4159
Takefuji, Mikito et al.RhoGEF12 controls cardiac remodeling by integrating G protein- and integrindependent signaling cascadesJournal of Experimental Medicine2013ISSN 0022-1007
Gil-Henn, H. et al.Arg/Abl2 promotes invasion and attenuates proliferation of breast cancer in vivoOncogene2013ISSN 0950-9232
Liu, Yao et al.Actin polymerization-dependent increase in synaptic Arc/Arg3.1 expression in the Amygdala is crucial for the expression of aversive memory associated with drug withdrawalJournal of Neuroscience2012ISSN 0270-6474
Padró, Teresa et al.LDL-induced impairment of human vascular smooth muscle cells repair function is reversed by HMG-CoA reductase inhibitionPLoS ONE2012ISSN 1932-6203
Ray, Anasuya et al.Hedgehog signaling pathway regulates the growth of ovarian cancer spheroid forming cellsInternational Journal of Oncology2011ISSN 1019-6439
Hook, Jeff et al.Functional identity of the gamma tropomyosin gene: Implications for embryonic development, reproduction and cell viabilityBioArchitecture2011ISSN 1949-100X
Romero, Ana M. et al.Chronic ethanol exposure alters the levels, assembly, and cellular organization of the actin cytoskeleton and microtubules in hippocampal neurons in primary cultureToxicological Sciences2010ISSN 1096-6080
Aguilar, Hector N. et al.Quantification of rapid myosin regulatory light chain phosphorylation using high-throughput in-cell western assays: Comparison to western immunoblotsPLoS ONE2010ISSN 1932-6203


Question 1:  Can I change the dilution volume of the experimental lysate being assayed?

Answer 1:  Yes, different volumes of lysate can be used to quantify protein levels.  The equation to determine protein concentration in µg/ml is: OD reading x dilution factor x 100 µg/ml/cm if a small volume (1.0 ml) cuvette is used.  If a 96 well plate is used, the same equation is used with this single modification: 125 µg/ml/cm is the value used rather than 100 µg/ml/cm.


Question 2:  Can this assay be performed using 96 well plates?

Answer 2: Yes, the protein assay can be performed in either small volume cuvettes (1.0 ml) or a 96 well plate.  If a 96 well plate is used, the equation to determine protein concentration in µg/ml is: OD reading x dilution factor x 125 µg/ml/cm.  



If you have any questions concerning this product, please contact our Technical Service department at