Precision Red Advanced Protein Assay: 1x stock

Precision Red Advanced Protein Assay: 1x stock
$0.00

Product uses include measuring protein in

  • Cell extracts
  • Serum samples
  • Medium to high protein concentration solutions
  • Protein requiring GLP and higher standards
  • Purified proteins and antibodies

Material
The Precision Red Advanced Protein Assay Reagent is designed to optimize the speed and accuracy of protein measurement. The reagent combines the useful properties of low protein to protein variance (See Fig 1) an
 d a wide protein concentration for a robust assay. The assay can measure accurately protein concentrations ranging from 0.25 to 50 mg/ml and is detergent compatible. A simple one step procedure results in a red to blue color change within 1 min, which can be recognized by measuring absorbance at 600 nm.

This reagent is so reproducible with a wide range of proteins and protein concentrations that it is employed to accurately determine the concentration in all of Cytoskeleton, Inc's protein products.

adv-variance

Figure 1: The low protein to protein variance with ADV01 and ADV02. A comparison of ADV01, ADV02, Bradford and Lowry assays on five different proteins.

Method
The Precision Red Advanced Protein Assay makes it very easy and quick to measure prottein concentrations. A general method looks as follows:

  1. Mix protein solution with ADV02 reagent (e.g. 10 µl sample with 1.0 ml ADV02)
  2. Incubate 1 min
  3. Read absorbance at 600 nm

Detergent and chemical compatibility
The Precision Red Advanced Protein Assay is a highly stable assay and it tolerates a wide range of reagents. See the Advanced Protein Assay reagent compatibility page for a full list of reagents that have been tested.

Using the Precision Red Advanced Protein Assay in cell extracts
Since the Precision Red Advanced Protein Assay is highly compatible with non-ionic detergents (see the ADV Chemical tolerance page), this reagent is ideal for measuring protein concentrations in cell and tissue extracts. Furthermore, since protein concentration can be measured after only 1 min of incubation with the reagent, it allows the user to proceed with the experiment quicker than any other protein assay, something that can be of big importance when working with labile cell extracts.

Cytoskeleton, Inc. recommends using ADV02 for all our kits and assays that involve cell or tissue lysis. In the G-LISA™ small G-protein activation kits, we have even included this reagent to ensure that the user gets the most accurate and quickest possible measurement of the protein concentration in the cell lysates generated.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

AuthorTitleJournalYearArticle Link
Fongsaran, Chanida et al.Arbovirus infection increases the risk for the development of neurodegenerative disease pathology in the murine modelBrain, Behavior, & Immunity - Health2024
Fongsaran, Chanida et al.VEEV TC-83 Triggers Dysregulation of the Tryptophan–Kynurenine Pathway in the Central Nervous System That Correlates with Cognitive Impairment in Tg2576 MicePathogens2024
Hung, Christy et al.APP antisense oligonucleotides reduce amyloid-β aggregation and rescue endolysosomal dysfunction in Alzheimer’s diseaseBrain2024
Hung, Christy et al.Elevated 4R tau contributes to endolysosomal dysfunction and neurodegeneration in VCP-related frontotemporal dementiaBrain2024
Saurat, Nathalie et al.Genome-wide CRISPR screen identifies neddylation as a regulator of neuronal aging and AD neurodegenerationCell Stem Cell2024
Velázquez-Vega, Luis E. et al.Efficacy and delivery strategies of the dual Rac/Cdc42 inhibitor MBQ-167 in HER2 overexpressing breast cancerTranslational Oncology2024
Flak, Magdalena B. et al.Deletion of macrophage Gpr101 disrupts their phenotype and function dysregulating host immune responses in sterile and infectious inflammationBiochemical Pharmacology2023
Prateeksha, Prateeksha et al.Secretome of Dental Pulp-Derived Stem Cells Reduces Inflammation and Proliferation of Glioblastoma Cells by Deactivating Mapk-Akt PathwayDiseases & research2023
Ortiz-Soto, Gabriela et al.Metadherin Regulates Inflammatory Breast Cancer Invasion and MetastasisInternational Journal of Molecular Sciences2023
Lopes, Nayara A. et al.Environmental Enrichment Promotes Transgenerational Programming of Uterine Inflammatory and Stress Markers Comparable to Gestational Chronic Variable StressInternational Journal of Molecular Sciences2023
Vicente-Rodríguez, Laura C. et al.The FMRF-NH2 gated sodium channel of Biomphalaria glabrata: Localization and expression following infection by Schistosoma mansoniPLOS Neglected Tropical Diseases2023
Datta, Gaurav et al.17⍺-Estradiol Protects against HIV-1 Tat-Induced Endolysosome Dysfunction and Dendritic Impairments in NeuronsCells2023
Dvorak, Nolan M. et al.TNFR1 signaling converging on FGF14 controls neuronal hyperactivity and sickness behavior in experimental cerebral malariaJournal of Neuroinflammation2023
Munni, Yeasmin Akter et al.Differential Effects of the Processed and Unprocessed Garlic (Allium sativum L.) Ethanol Extracts on Neuritogenesis and Synaptogenesis in Rat Primary Hippocampal NeuronsInternational Journal of Molecular Sciences2023
Ali, Yusuf et al.Mammalian Target of Rapamycin Inhibition Decreases Angiotensin II-Induced Steroidogenesis in HAC15 Human Adrenocortical Carcinoma CellsEndocrinology2022
Cruz-Nova, Pedro et al.131I-C19 Iodide Radioisotope and Synthetic I-C19 Compounds as K-Ras4B–PDE6δ Inhibitors: A Novel Approach against Colorectal Cancer—Biological Characterization, Biokinetics and DosimetryMolecules2022
Hassin, Ori et al.Different hotspot p53 mutants exert distinct phenotypes and predict outcome of colorectal cancer patientsNature Communications2022
Xu, Weiyi et al.Paxillin promotes breast tumor collective cell invasion through maintenance of adherens junction integrityMolecular biology of the cell2022
Shields, Mario A. et al.Gα13 loss in Kras/Tp53 mouse model of pancreatic tumorigenesis promotes tumors susceptible to rapamycinCell Reports2022
Datta, Gaurav et al.Endolysosome Localization of ERα Is Involved in the Protective Effect of 17α-Estradiol against HIV-1 gp120-Induced Neuronal InjuryThe Journal of neuroscience : the official journal of the Society for Neuroscience2021
Hryniewicz-Jankowska, Anita et al.Article the effect of neddylation inhibition on inflammation-induced mmp9 gene expression in esophageal squamous cell carcinomaInternational Journal of Molecular Sciences2021
Le, Anh Hoang et al.Cyri-a limits invasive migration through macropinosome formation and integrin uptake regulationJournal of Cell Biology2021
Nehme, Jamil et al.High dietary protein and fat contents exacerbate hepatic senescence and SASP in miceFEBS Journal2021
Liao, Liang et al.Disrupting RhoA activity by blocking Arhgef3 expression mitigates microglia-induced neuroinflammation post spinal cord contusionJournal of Neuroimmunology2021
Dolmatova, Elena V et al.Endothelial Poldip2 regulates sepsis-induced lung injury via Rho pathway activationCardiovascular Research2021
Rojas-Colón, Luis A. et al.4R-cembranoid confers neuroprotection against LPS-induced hippocampal inflammation in miceJournal of Neuroinflammation2021
Bordeleau, Francois et al.Tissue transglutaminase 2 regulates tumor cell tensional homeostasis by increasing contractilityJournal of Cell Science2020
Papalazarou, Vassilis et al.The creatine–phosphagen system is mechanoresponsive in pancreatic adenocarcinoma and fuels invasion and metastasisNature Metabolism2020
Dias Gomes, Martim et al.Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrityNature Communications2019
Stephens, Matthew et al.Mesenteric lymphatic alterations observed during DSS induced intestinal inflammation are driven in a TLR4-PAMP/DAMP discriminative mannerFrontiers in Immunology2019
Cederquist, Gustav Y. et al.Specification of positional identity in forebrain organoidsNature Biotechnology2019
Tedeschi, Andrea et al.ADF/Cofilin-Mediated Actin Turnover Promotes Axon Regeneration in the Adult CNSNeuron2019
Kikuchi, Daniel S. et al.Poldip2 mediates blood-brain barrier disruption in a model of sepsis-associated encephalopathyJournal of Neuroinflammation2019
Umesalma, Shaikamjad et al.RABL6A inhibits tumor-suppressive PP2A/AKT signaling to drive pancreatic neuroendocrine tumor growthJournal of Clinical Investigation2019
Paonessa, Francesco et al.Microtubules Deform the Nuclear Membrane and Disrupt Nucleocytoplasmic Transport in Tau-Mediated Frontotemporal DementiaCell Reports2019
Juin, Amelie et al.N-WASP Control of LPAR1 Trafficking Establishes Response to Self-Generated LPA Gradients to Promote Pancreatic Cancer Cell MetastasisDevelopmental Cell2019
Cruz-Nova, Pedro et al.The small organic molecule C19 binds and strengthens the KRAS4b-PDEδ complex and inhibits growth of colorectal cancer cells in vitro and in vivo 11 Medical and Health Sciences 1112 Oncology and CarcinogenesisBMC Cancer2018
Casique-Aguirre, Diana et al.KRas4B-PDE6δ complex stabilization by small molecules obtained by virtual screening affects Ras signaling in pancreatic cancer 06 Biological Sciences 0601 Biochemistry and Cell BiologyBMC Cancer2018
McQueeney, Kelley E. et al.Targeting ovarian cancer and endothelium with an allosteric PTP4A3 phosphatase inhibitorOncotarget2018
Zhu, Yili et al.An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated ProteinBio-Protocol2018
Proto, Jonathan D. et al.Regulatory T Cells Promote Macrophage Efferocytosis during Inflammation ResolutionImmunity2018
Mangiatordi, Giuseppe Felice et al.Automated identification of structurally heterogeneous and patentable antiproliferative hits as potential tubulin inhibitorsChemical Biology and Drug Design2018
Meiring, Joyce C.M. et al.Co-polymers of Actin and Tropomyosin Account for a Major Fraction of the Human Actin CytoskeletonCurrent Biology2018
Alhasani, Reem Hasaballah et al.Gypenosides protect retinal pigment epithelium cells from oxidative stressFood and Chemical Toxicology2018
Patnaik, Sarita Rani et al.RPGR protein complex regulates proteasome activity and mediates store-operated calcium entryOncotarget2018
Borin, Mirta et al.Rac1 activation links tau hyperphosphorylation and Aß dysmetabolism in Alzheimer's diseaseActa Neuropathologica Communications2018
Zhang, Xiaoqing et al.Alterations of MEK1/2-ERK1/2, IFNγ and Smad2/3 associated Signalling pathways during cryopreservation of ASCs affect their differentiation towards VSMC-like cellsStem Cell Research2018
Horita, Henrick et al.Utilizing a comprehensive immunoprecipitation enrichment system to identify an endogenous post-translational modification profile for target proteinsJournal of Visualized Experiments2018
Hendricks, Louie et al.Milk fat globule-epidermal growth factor-factor VIII–derived peptide MSP68 is a cytoskeletal immunomodulator of neutrophils that inhibits Rac1Journal of Surgical Research2017
Huang, Jia Lin et al.Lipoprotein-biomimetic nanostructure enables efficient targeting delivery of siRNA to Ras-activated glioblastoma cells via macropinocytosisNature Communications2017
Albalawi, Aishah et al.Protective effect of carnosic acid against acrylamide-induced toxicity in RPE cellsFood and Chemical Toxicology2017
Mangiatordi, Giuseppe Felice et al.Novel chemotypes targeting tubulin at the colch***** binding site and unbiasing P-glycoproteinEuropean Journal of Medicinal Chemistry2017
Chen, Lihua et al.Smooth muscle-alpha actin inhibits vascular smooth muscle cell proliferation and migration by inhibiting rac1 activityPLoS ONE2016
Zan, G. Y. et al.p38 mitogen-activated protein kinase activation in amygdala mediates κ opioid receptor agonist U50,488H-induced conditioned place aversionNeuroscience2016
Lampi, Marsha C. et al.Simvastatin ameliorates matrix stiffness-mediated endothelial monolayer disruptionPLoS ONE2016
Chen, Weihua et al.High RhoA expression at the tumor front in clinically localized prostate cancer and association with poor tumor differentiationOncology Letters2016
Castillo-Pichardo, Linette et al.Dietary grape polyphenol resveratrol increases mammary tumor growth and metastasis in immunocompromised mice2013
Zilberter, Misha et al.Dietary energy substrates reverse early neuronal hyperactivity in a mouse model of Alzheimer's diseaseJournal of Neurochemistry2013
Takefuji, Mikito et al.RhoGEF12 controls cardiac remodeling by integrating G protein- and integrindependent signaling cascadesJournal of Experimental Medicine2013
Gil-Henn, H. et al.Arg/Abl2 promotes invasion and attenuates proliferation of breast cancer in vivoOncogene2013
Liu, Yao et al.Actin polymerization-dependent increase in synaptic Arc/Arg3.1 expression in the Amygdala is crucial for the expression of aversive memory associated with drug withdrawalJournal of Neuroscience2012
Padró, Teresa et al.LDL-induced impairment of human vascular smooth muscle cells repair function is reversed by HMG-CoA reductase inhibitionPLoS ONE2012
Ray, Anasuya et al.Hedgehog signaling pathway regulates the growth of ovarian cancer spheroid forming cellsInternational Journal of Oncology2011
Hook, Jeff et al.Functional identity of the gamma tropomyosin gene: Implications for embryonic development, reproduction and cell viabilityBioArchitecture2011
Romero, Ana M. et al.Chronic ethanol exposure alters the levels, assembly, and cellular organization of the actin cytoskeleton and microtubules in hippocampal neurons in primary cultureToxicological Sciences2010
Aguilar, Hector N. et al.Quantification of rapid myosin regulatory light chain phosphorylation using high-throughput in-cell western assays: Comparison to western immunoblotsPLoS ONE2010

 

Question 1:  Can I change the dilution volume of the experimental lysate being assayed?

Answer 1:  Yes, different volumes of lysate can be used to quantify protein levels.  The equation to determine protein concentration in µg/ml is: OD reading x dilution factor x 100 µg/ml/cm if a small volume (1.0 ml) cuvette is used.  If a 96 well plate is used, the same equation is used with this single modification: 125 µg/ml/cm is the value used rather than 100 µg/ml/cm.

 

Question 2:  Can this assay be performed using 96 well plates?

Answer 2: Yes, the protein assay can be performed in either small volume cuvettes (1.0 ml) or a 96 well plate.  If a 96 well plate is used, the equation to determine protein concentration in µg/ml is: OD reading x dilution factor x 125 µg/ml/cm.  

 

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com