Product Uses Include
The Ras superfamily of small GTPases (such as Ras, Rho, Rab, Arf and Ran proteins) serve as binary switches cycling between a GDP-bound “OFF state” and a GTP-bound “ON state”. In cells, the cycling between the two states is mainly controlled by two types of regulatory proteins: the activating Guanine nucleotide Exchange Factors (GEFs) and the inactivating GTPase Activating Proteins (GAPs).
GEFs function by catalyzing the exchange of nucleotide on their target GTPases. The GEF will bind to a nucleotide-bound GTPase, which causes the bound nucleotide to be released, thus resulting in a nucleotide free GEF-GTPase reaction intermediate. This nucleotide free complex will then take up a new nucleotide after which the GEF is released from the GTPase. Because GEFs typically have a higher affinity for GDP-bound GTPases than for the corresponding GTP-bound GTPase and the intracellular ratio of GTP to GDP is about 10:1, GEFs will drive the exchange from GDP-bound GTPases to GTP-bound GTPases
The fluorophore based assay in this kit is suitable for measuring nucleotide exchange on GTPases in either 96-well or 384-well format. The kit measures the uptake of the fluorescent nucleotide analog N-methylanthraniloyl-GTP (N-MAR-GTP) into GTPases. The uptake can be measured due to the spectroscopic difference between free and GTPase-bound N-MAR-GTP. As N-MAR-GTP gets bound in the nucleotide binding pocket of a GTPase, its fluorescence (ex: 360 nm, em: 440 nm) increases dramatically (1, 2). Therefore, the enhancement of N-MAR-GTP fluorescent intensity in the presence of a small GTPase indicates nucleotide uptake (or exchange for already bound nucleotide) by the GTPase.
This kit contains all the buffers and reagents needed for the assay, a 96 and a 384-well plate, as well as human Cdc42, Rac1 and RhoA GTPases and the GEF domain of Dbs as a positive control GEF. Dbs is an effective GEF for Cdc42 and RhoA but shows only very low GEF activity for Rac1 (see Figs 1 & 2). The kit provides enough material for 20 assays for each GTPase in 96-well format or 100 assays for each GTPase in 384-well format.
While the kit comes with Cdc42, Rac1 and RhoA proteins, it can also be used for any other Ras superfamily GTPase. See our G-protein product family web page for other GTPases available from Cytoskeleton.
For a kit to measure GAP activity, see Cat. # BK105
The kit contains enough materials for 60 assays in 96-well format or 300 assays in 384-well format. The following components are included:
The exchange activity of Dbs on Cdc42, RhoA and Rac1 was measured in 96-well and 384-well format (Fig 1 & 2)
Figure 1. Dbs exchange activity for Cdc42, RhoA and Rac1 in 96-well half area plate format
Figure 2. Dbs exchange activity for Cdc42, RhoA and Rac1 in 384-well plate format
|Xu, Yangfan et al.||A small Rho GTPase OsRacB is required for pollen germination in rice||Development Growth and Differentiation||2022||ISSN 1440-169X|
|Bharadwaj, Ravi et al.||An atypical EhGEF regulates phagocytosis in Entamoeba histolytica through EhRho1||PLoS Pathogens||2021||ISSN 1553-7374|
|Giubilaro, Jenna et al.||Discovery of a dual Ras and ARF6 inhibitor from a GPCR endocytosis screen||Nature Communications||2021||ISSN 2041-1723|
|Eshraghi, Mehdi et al.||RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease||Science advances||2020||ISSN 2375--2548|
|Limzerwala, Jazeel F. et al.||FoxM1 insufficiency hyperactivates Ect2–RhoA–mDia1 signaling to drive cancer||Nature Cancer||2020||ISSN 2662-1347|
|Miao, Hui et al.||Cell ratcheting through the Sbf RabGEF directs force balancing and stepped apical constriction||Journal of Cell Biology||2019||ISSN 1540-8140|
|Singh, Nikhlesh K. et al.||P115 RhoGEF activates the Rac1 GTPase signaling cascade in MCP1 chemokine-induced vascular smooth muscle cell migration and proliferation||Journal of Biological Chemistry||2017||ISSN 1083-351X|
|Rosenberg, Brian J. et al.||Phosphorylated cortactin recruits Vav2 guanine nucleotide exchange factor to activate Rac3 and promote invadopodial function in invasive breast cancer cells||Molecular Biology of the Cell||2017||ISSN 1939-4586|
|Huang, Guorui et al.||WBSCR16 Is a Guanine Nucleotide Exchange Factor Important for Mitochondrial Fusion||Cell Reports||2017||ISSN 2211-1247|
|Barreira, María et al.||The C-terminal SH3 domain contributes to the intramolecular inhibition of Vav family proteins||Science Signaling||2014||ISSN 1937-9145|
|Schulz, Alexander et al.||Merlin isoform 2 in neurofibromatosis type 2-associated polyneuropathy||Nature Neuroscience||2013||ISSN 1097-6256|
|Moey, Melissa et al.||Ginseng (Panax quinquefolius) attenuates leptin-induced cardiac hypertrophy through inhibition of p115Rho guanine nucleotide exchange factor-RhoA/Rho-associated, coiled-coil containing protein kinase-dependent mitogen-activated protein kinase pathway activation||The Journal of pharmacology and experimental therapeutics||2011||ISSN 1521--0103|
|Guilluy, Christophe et al.||The Rho exchange factor Arhgef1 mediates the effects of angiotensin II on vascular tone and blood pressure||Nature medicine||2010||ISSN 1546--170X|
|Oliver, A. W. et al.||The HPV16 E6 binding protein Tip-1 interacts with ARHGEF16, which activates Cdc42||British Journal of Cancer 2011 104:2||2010||ISSN 1532--1827|
|Arbeloa, Ana et al.||EspM2 is a RhoA guanine nucleotide exchange factor||Cellular Microbiology||2010||ISSN 1462-5814|
|Li, Siwei et al.||PLC-γ1 and Rac1 Coregulate EGF-Induced Cytoskeleton Remodeling and Cell Migration||Molecular Endocrinology||2009||ISSN 0888--8809|
|Kim, Su Jin et al.||Dipeptidyl peptidase IV inhibition with MK0431 improves islet graft survival in diabetic NOD mice partially via T-cell modulation||Diabetes||2009||ISSN 1939--327X|
|Nakada-Tsukui, Kumiko et al.||Phosphatidylinositol-phosphates mediate cytoskeletal reorganization during phagocytosis via a unique modular protein consisting of RhoGEF/DH and FYVE domains in the parasitic protozoon Entamoeba histolytica||Cellular Microbiology||2009||ISSN 1462-5814|
Question 1: What is the best way to measure GEF activity from a cell lysate?
Answer 1: First, the GEF protein must be immunoprecipitated from the cell lysate with either an antibody to the protein or to a tag (His, GST, etc.) that has been conjugated to the protein of interest. After eluting your protein of interest from the beads, the protein’s GEF activity can be measured using Cytoskeleton’s RhoGEF Exchange Assay Biochem Kit (Cat. # BK100). Cytoskeleton Inc. has developed a mant fluorophore-based GEF assay designed for characterizing GEFs and identifying GEF inhibitors. This kit contains human Cdc42 (Cat. # CD01), Rac1 (Cat. # RC01) and RhoA (Cat. # RH01) proteins and the GEF domain of Dbs (Cat. # GE01) as a positive control GEF for Cdc42 and RhoA. The kit also comes with a 384-well and 96-well plate along with exchange buffer that contains the mant-GTP. Once bound to GTPases, the fluorophore emission intensity of mant-GTP increases approximately 1.1 to 2.0 fold depending on the protein. Therefore, the enhancement of fluorescent intensity in the presence of small GTPases and GEFs will reflect the respective GEF activities of known or unknown proteins. We recommend titrating the concentration of your GEF protein to optimize its activity. We suggest a titration range of 0.01-1 µM. A citation that used our GEF assay kit with an immunoprecipitated protein is Kakiashvili et al., 2009. GEF-H1 mediates tumor necrosis factor-alpha-induced Rho activation and myosin phosphorylation: role in the regulation of tubular paracellular permeability. J. Biol. Chem. 284, 11454-11466.
Question 2: I want to use the RhoGEF Exchange Assay kit with a 384 well plate. Is this possible?
Answer 2: Yes, Cytoskeleton’s mant fluorophore based GEF assay (Cat. # BK100) is suitable for a 384 well high-throughput screen format. Up to 130 reactions per GTPase can be achieved if a 384-well low volume black round bottom plate (Corning Cat# 3676) is used. This type of 384 well plate is strongly recommended due to the greater signal/noise ratio. Do not use clear plates since that will give you significant background noise. We provide a 384 well plate and a protocol for this HTS format in the manual that comes with the kit.
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