Protease inhibitor cocktail

Protease inhibitor cocktail
$0.00

Product Uses Include

  • Inhibition of a wide range of proteases.
  • Popular Activation Assay Component

Material
Protease Inhibitor Cocktail is supplied as a white lyophilized powder. The cocktail contains the following protease inhibitors, Pepstatin A, Leupeptin, Benzamidine, Na-p-tosyl-L-arginine methyl ester (TAME). At 1X final working concentration the inhibitors are at 1 µM, 1.5 µM, 1 mM and 0.4 mM respectively.

Mode of Action
pepstatin A Reversibly inhibits some aspartic proteases
Leupeptin Reversibly inhibits trypsin like serine and some cysteine proteases
Benzamidine Inhibits serine proteases
TAME Inhibits arginine proteases, a thrombin and trypsin inhibitor

Storage and Reconstitution
Lyophilized Protease Inhibitor Cocktail is stable at 4°C desiccated (<10% humidity) for 1 year.
Each tube of Protease Inhibitor Cocktail should be resuspended in 1ml of DMSO (dimethyl sulfoxide) to give a 100X strength stock. The reconstituted stock can be stored at 4°C for up to 6 months or at -70°C for up to 1 year.

Related Products
This protease inhibitor cocktail is used in the following Cytoskeleton Activation Assays:

  • Ras activation assay Biochem kit (Cat. # BK008)
  • Cdc42 activation assay Biochem kit (Cat. # BK034)
  • Rac activation assay Biochem kit (Cat. # BK035)
  • RhoA activation assay Biochem kit (Cat. # BK036)
  • G-actin : F-actin in vivo assay Biochem kit (Cat. # BK037)
  • Microtubule : tubulin in vivo assay Biochem kit (Cat. # BK038)
  • RhoA G-LISA™ Activation Assay Kit (luminescence) (Cat. # BK121)
  • RhoA G-LISA™ Activation Assay Kit (colorimetric) (Cat. # BK124)
  • Rac1,2,3 G-LISA™ Activation Assay Kit (colorimetric) (Cat. # BK125)
  • Rac1 G-LISA™ Activation Assay Kit (luminescence) (Cat. # BK126)
  • Cdc42 G-LISA™ Activation Assay (Cat. # BK127)
  • Rac1 G-LISA™ Activation Assay (Colorimetric Based) (Cat. # BK128)
  • RalA G-LISA™ Activation Assay (Colorimetric Based) (Cat. # BK129)

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

AuthorTitleJournalYearArticle Link
Yau, Eric et al.SP-R210 isoforms of Myosin18A modulate endosomal sorting and recognition of influenza A virus infection in macrophagesMicrobes and Infection2023ISSN 1286--4579
Wang, Yuan et al.MTSS1 curtails lung adenocarcinoma immune evasion by promoting AIP4-mediated PD-L1 monoubiquitination and lysosomal degradationCell Discovery 2023 9:12023ISSN 2056--5968
Saldías, María Paz et al.The Cytoplasmic Region of SARAF Reduces Triple-Negative Breast Cancer Metastasis through the Regulation of Store-Operated Calcium EntryInternational Journal of Molecular Sciences 2023, Vol. 24, Page 53062023ISSN 1422--0067
Román-Fernández, Alvaro et al.Spatial regulation of the glycocalyx component podocalyxin is a switch for prometastatic functionScience Advances2023ISSN 2375-2548
Liang, Chen et al.Carboxypeptidase E Independently Changes Microtubule Glutamylation, Dendritic Branching, and Neuronal MigrationASN Neuro2022ISSN 1759-0914
Zhang, Yan et al.Different changes in pre- and postsynaptic components in the hippocampal CA1 subfield after transient global cerebral ischemiaBrain Structure and Function2022ISSN 1863-2661
Ma, Yuanyuan et al.Ror2-mediated non-canonical Wnt signaling regulates Cdc42 and cell proliferation during tooth root developmentDevelopment (Cambridge)2021ISSN 1477-9129
Fernandez, Christian et al.TMPRSS11a is a novel age-altered, tissue specific regulator of migration and wound healingFASEB Journal2021ISSN 1530-6860
Berganza, Eider et al.A multiplexed phospholipid membrane platform for curvature sensitive protein screeningNanoscale2021ISSN 2040-3372
Gu, Chunming et al.Identification of berberine as a novel drug for the treatment of multiple myeloma via targeting UHRF1BMC Biology2020ISSN 1741-7007
Rivas, José et al.KCTD5, a novel TRPM4-regulatory protein required for cell migration as a new predictor for breast cancer prognosisFASEB Journal2020ISSN 1530-6860
Park, Jin Suk et al.Mechanical regulation of glycolysis via cytoskeleton architectureNature2020ISSN 1476-4687
Sodhi, Chhinder P. et al. A Dynamic Variation of Pulmonary ACE2 Is Required to Modulate Neutrophilic Inflammation in Response to Pseudomonas aeruginosa Lung Infection in Mice The Journal of Immunology2019ISSN 0022--1767
Blanco, Constanza et al.EB1- and EB2-dependent anterograde trafficking of TRPM4 regulates focal adhesion turnover and cell invasionFASEB Journal2019ISSN 1530-6860
Dér, Bálint et al.NK2 receptor-mediated detrusor muscle contraction involves Gq/11-dependent activation of voltage-dependent Ca2+ channels and the RhoA-Rho kinase pathwayAmerican Journal of Physiology - Renal Physiology2019ISSN 1522-1466
Martin, Jennifer A. et al.A novel role for the actin-binding protein drebrin in regulating opiate addictionNature Communications2019ISSN 2041-1723
Tong, Jing et al.Qiliqiangxin Rescues Mouse Cardiac Function by Regulating AGTR1/TRPV1-Mediated Autophagy in STZ-Induced Diabetes MellitusCellular Physiology and Biochemistry2018ISSN 1421-9778
Riquelme, Denise et al.Subcellular localization and activity of TRPM4 in medial prefrontal cortex layer 2/3Frontiers in Cellular Neuroscience2018ISSN 1662-5102
Weise-Cross, Laura et al.Actin polymerization contributes to enhanced pulmonary vasoconstrictor reactivity after chronic hypoxiaAmerican Journal of Physiology - Heart and Circulatory Physiology2018ISSN 1522-1539
Horita, Henrick et al.Utilizing a comprehensive immunoprecipitation enrichment system to identify an endogenous post-translational modification profile for target proteinsJournal of Visualized Experiments2018ISSN 1940-087X
Horita, Henrick et al.Utilizing optimized tools to investigate PTM crosstalk: Identifying potential PTM crosstalk of acetylated mitochondrial proteinsProteomes2018ISSN 2227-7382
Pérez-Núñez, Ramón et al.Overexpressed Down Syndrome Cell Adhesion Molecule (DSCAM) Deregulates P21-Activated Kinase (PAK) Activity in an In Vitro Neuronal Model of Down Syndrome: Consequences on Cell Process Formation and ExtensionNeurotoxicity Research2016ISSN 1476-3524
Jia, Hongpeng et al.Pulmonary Epithelial TLR4 Activation Leads to Lung Injury in Neonatal Necrotizing EnterocolitisThe Journal of Immunology2016ISSN 0022--1767
Wang, J. et al.Erratum: The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus (Acta Pharmacol Sin (2013) 34 (811-8) Doi: 10.1038/aps.2013.3 )Acta Pharmacologica Sinica2014ISSN 1745-7254
Munnamalai, Vidhya et al.Bidirectional interactions between NOX2-type NADPH oxidase and the F-actin cytoskeleton in neuronal growth conesJournal of neurochemistry2014ISSN 1471--4159
Vindin, Howard et al.Validation of an algorithm to quantify changes in actin cytoskeletal organizationJournal of biomolecular screening2014ISSN 1552--454X
Tan, Hong et al.Fluid flow forces and rhoA regulate fibrous development of the atrioventricular valvesDevelopmental Biology2013ISSN 1095-564X
Yang, Jian et al.Inhibition of farnesyl pyrophosphate synthase attenuates angiotensin II-induced cardiac hypertrophy and fibrosis in vivoInternational Journal of Biochemistry and Cell Biology2013ISSN 1357-2725

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