• Fluorogenic stain for actin filaments in live cells
• Fluorogenic stain for actin filaments in paraformaldehyde (PF) fixed cells
• Supports multicolor imaging

SPY™555-actin probe comprises SPY™555 dye conjugated to the actin-binding natural product jasplakinolide. Its fluorogenic nature allows the labelling of F-actin in live and fixed cells with high specificity and low background.

Key features

• Ab: 555 nm, Em: 580 nm
• Cell-permeable
• Works on live or fixed cells
• Fluorogenic character: enhanced fluorescence when bound to F-actin
• Compatible with widefield, confocal, SIM, and STED imaging

The biological activity of CY-SC202 is assessed by the ability of the probe to efficiently label actin filaments in live HeLa cell culture. After a wash step, cell staining is visible for several hours. If performing longer term time-laps readings it is recommended to keep the probe in the image media with no wash step.