Protease inhibitor cocktail

Protease inhibitor cocktail
$0.00

Product Uses Include

  • Inhibition of a wide range of proteases.
  • Popular Activation Assay Component

Material
Protease Inhibitor Cocktail is supplied as a white lyophilized powder. The cocktail contains the following protease inhibitors, Pepstatin A, Leupeptin, Benzamidine, Na-p-tosyl-L-arginine methyl ester (TAME). At 1X final working concentration the inhibitors are at 1 µM, 1.5 µM, 1 mM and 0.4 mM respectively.

Mode of Action
pepstatin A Reversibly inhibits some aspartic proteases
Leupeptin Reversibly inhibits trypsin like serine and some cysteine proteases
Benzamidine Inhibits serine proteases
TAME Inhibits arginine proteases, a thrombin and trypsin inhibitor

Storage and Reconstitution
Lyophilized Protease Inhibitor Cocktail is stable at 4°C desiccated (<10% humidity) for 1 year.
Each tube of Protease Inhibitor Cocktail should be resuspended in 1ml of DMSO (dimethyl sulfoxide) to give a 100X strength stock. The reconstituted stock can be stored at 4°C for up to 6 months or at -70°C for up to 1 year.

Related Products
This protease inhibitor cocktail is used in the following Cytoskeleton Activation Assays:

  • Ras activation assay Biochem kit (Cat. # BK008)
  • Cdc42 activation assay Biochem kit (Cat. # BK034)
  • Rac activation assay Biochem kit (Cat. # BK035)
  • RhoA activation assay Biochem kit (Cat. # BK036)
  • G-actin : F-actin in vivo assay Biochem kit (Cat. # BK037)
  • Microtubule : tubulin in vivo assay Biochem kit (Cat. # BK038)
  • RhoA G-LISA™ Activation Assay Kit (luminescence) (Cat. # BK121)
  • RhoA G-LISA™ Activation Assay Kit (colorimetric) (Cat. # BK124)
  • Rac1,2,3 G-LISA™ Activation Assay Kit (colorimetric) (Cat. # BK125)
  • Rac1 G-LISA™ Activation Assay Kit (luminescence) (Cat. # BK126)
  • Cdc42 G-LISA™ Activation Assay (Cat. # BK127)
  • Rac1 G-LISA™ Activation Assay (Colorimetric Based) (Cat. # BK128)
  • RalA G-LISA™ Activation Assay (Colorimetric Based) (Cat. # BK129)

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

AuthorTitleJournalYearArticle Link
Gao, Da Peng et al.Chronic Alcohol Exposure Alters the Levels and Assembly of the Actin Cytoskeleton and Microtubules in the Adult Mouse HippocampusJournal of Integrative Neuroscience2024
Ha, Cuong P. et al.Humanin activates integrin αV–TGFβ axis and leads to glioblastoma progressionCell Death & Disease2024
Otani, Takahito et al.The role of adhesion molecules in osteocalcin-induced effects on glucose and lipid metabolism in adipocytesBiochimica et Biophysica Acta (BBA) - Molecular Cell Research2024
Hotani, Takuma et al.Involvement of Protein Kinase R in Double-Stranded RNA-Induced Proteasomal Degradation of Hypoxia Inducible Factor-1αInflammation2023
Chen, Shanlin et al.Aloe-emodin targets multiple signaling pathways by blocking ubiquitin-mediated degradation of DUSP1 in nasopharyngeal carcinoma cellsPhytotherapy Research2023
Yau, Eric et al.SP-R210 isoforms of Myosin18A modulate endosomal sorting and recognition of influenza A virus infection in macrophagesMicrobes and Infection2023
Wang, Yuan et al.MTSS1 curtails lung adenocarcinoma immune evasion by promoting AIP4-mediated PD-L1 monoubiquitination and lysosomal degradationCell Discovery 2023
Saldías, María Paz et al.The Cytoplasmic Region of SARAF Reduces Triple-Negative Breast Cancer Metastasis through the Regulation of Store-Operated Calcium EntryInternational Journal of Molecular Sciences2023
Román-Fernández, Alvaro et al.Spatial regulation of the glycocalyx component podocalyxin is a switch for prometastatic functionScience Advances2023
Liang, Chen et al.Carboxypeptidase E Independently Changes Microtubule Glutamylation, Dendritic Branching, and Neuronal MigrationASN Neuro2022
Zhang, Yan et al.Different changes in pre- and postsynaptic components in the hippocampal CA1 subfield after transient global cerebral ischemiaBrain Structure and Function2022
Ma, Yuanyuan et al.Ror2-mediated non-canonical Wnt signaling regulates Cdc42 and cell proliferation during tooth root developmentDevelopment (Cambridge)2021
Fernandez, Christian et al.TMPRSS11a is a novel age-altered, tissue specific regulator of migration and wound healingFASEB Journal2021
Berganza, Eider et al.A multiplexed phospholipid membrane platform for curvature sensitive protein screeningNanoscale2021
Gu, Chunming et al.Identification of berberine as a novel drug for the treatment of multiple myeloma via targeting UHRF1BMC Biology2020
Rivas, José et al.KCTD5, a novel TRPM4-regulatory protein required for cell migration as a new predictor for breast cancer prognosisFASEB Journal2020
Park, Jin Suk et al.Mechanical regulation of glycolysis via cytoskeleton architectureNature2020
Sodhi, Chhinder P. et al. A Dynamic Variation of Pulmonary ACE2 Is Required to Modulate Neutrophilic Inflammation in Response to Pseudomonas aeruginosa Lung Infection in Mice The Journal of Immunology2019
Blanco, Constanza et al.EB1- and EB2-dependent anterograde trafficking of TRPM4 regulates focal adhesion turnover and cell invasionFASEB Journal2019
Dér, Bálint et al.NK2 receptor-mediated detrusor muscle contraction involves Gq/11-dependent activation of voltage-dependent Ca2+ channels and the RhoA-Rho kinase pathwayAmerican Journal of Physiology - Renal Physiology2019
Martin, Jennifer A. et al.A novel role for the actin-binding protein drebrin in regulating opiate addictionNature Communications2019
Tong, Jing et al.Qiliqiangxin Rescues Mouse Cardiac Function by Regulating AGTR1/TRPV1-Mediated Autophagy in STZ-Induced Diabetes MellitusCellular Physiology and Biochemistry2018
Riquelme, Denise et al.Subcellular localization and activity of TRPM4 in medial prefrontal cortex layer 2/3Frontiers in Cellular Neuroscience2018
Weise-Cross, Laura et al.Actin polymerization contributes to enhanced pulmonary vasoconstrictor reactivity after chronic hypoxiaAmerican Journal of Physiology - Heart and Circulatory Physiology2018
Horita, Henrick et al.Utilizing a comprehensive immunoprecipitation enrichment system to identify an endogenous post-translational modification profile for target proteinsJournal of Visualized Experiments2018
Horita, Henrick et al.Utilizing optimized tools to investigate PTM crosstalk: Identifying potential PTM crosstalk of acetylated mitochondrial proteinsProteomes2018
Pérez-Núñez, Ramón et al.Overexpressed Down Syndrome Cell Adhesion Molecule (DSCAM) Deregulates P21-Activated Kinase (PAK) Activity in an In Vitro Neuronal Model of Down Syndrome: Consequences on Cell Process Formation and ExtensionNeurotoxicity Research2016
Jia, Hongpeng et al.Pulmonary Epithelial TLR4 Activation Leads to Lung Injury in Neonatal Necrotizing EnterocolitisThe Journal of Immunology2016
Wang, J. et al.Erratum: The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus (Acta Pharmacol Sin (2013) 34 (811-8) Doi: 10.1038/aps.2013.3 )Acta Pharmacologica Sinica2014
Munnamalai, Vidhya et al.Bidirectional interactions between NOX2-type NADPH oxidase and the F-actin cytoskeleton in neuronal growth conesJournal of neurochemistry2014
Vindin, Howard et al.Validation of an algorithm to quantify changes in actin cytoskeletal organizationJournal of biomolecular screening2014
Tan, Hong et al.Fluid flow forces and rhoA regulate fibrous development of the atrioventricular valvesDevelopmental Biology2013
Yang, Jian et al.Inhibition of farnesyl pyrophosphate synthase attenuates angiotensin II-induced cardiac hypertrophy and fibrosis in vivoInternational Journal of Biochemistry and Cell Biology2013

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