Protein Assays

Developed by scientists at Cytoskeleton, the Advanced Protein Assay (Cat. # ADV01) and the Precision Red Advanced Protein Assay (cat. # ADV02) are highly recommended for measuring purified protein and total cell protein alike. The advantages of ADV01 are the low detection limit (0.25 µg protein/ml sample) and the highly similar readings between the same amounts of different purified proteins. ADV02 is less sensitive but more compatible with detergents, and retains the same similarity between different purified proteins. In comparison to the Bradford assay these assays read different purified proteins with two fold greater accuracy which allows the user to rely on the simple A = Ecl  formula to convert the OD into a real protein concentration.

adv01-sds

For protein assay protocols, click here

For more information view ours datasheets, or contact Technical Support at tservice@cytoskeleton.com .

Protein Assay Product Citations

AuthorTitleJournalYearArticle Link
Xu, Weiyi et al.Paxillin promotes breast tumor collective cell invasion through maintenance of adherens junction integrityMolecular biology of the cell2022ISSN 1939-4586
Shields, Mario A. et al.Gα13 loss in Kras/Tp53 mouse model of pancreatic tumorigenesis promotes tumors susceptible to rapamycinCell Reports2022ISSN 2211-1247
Hryniewicz-Jankowska, Anita et al.Article the effect of neddylation inhibition on inflammation-induced mmp9 gene expression in esophageal squamous cell carcinomaInternational Journal of Molecular Sciences2021ISSN 1422-0067
Nehme, Jamil et al.High dietary protein and fat contents exacerbate hepatic senescence and SASP in miceFEBS Journal2021ISSN 1742-4658
Le, Anh Hoang et al.Cyri-a limits invasive migration through macropinosome formation and integrin uptake regulationJournal of Cell Biology2021ISSN 1540-8140
Datta, Gaurav et al.Endolysosome Localization of ERα Is Involved in the Protective Effect of 17α-Estradiol against HIV-1 gp120-Induced Neuronal InjuryThe Journal of neuroscience : the official journal of the Society for Neuroscience2021ISSN 1529-2401
Liao, Liang et al.Disrupting RhoA activity by blocking Arhgef3 expression mitigates microglia-induced neuroinflammation post spinal cord contusionJournal of Neuroimmunology2021ISSN 1872-8421
Rojas-Colón, Luis A. et al.4R-cembranoid confers neuroprotection against LPS-induced hippocampal inflammation in miceJournal of Neuroinflammation2021ISSN 1742-2094
Dolmatova, Elena V et al.Endothelial Poldip2 regulates sepsis-induced lung injury via Rho pathway activationCardiovascular Research2021ISSN 0008--6363
Bordeleau, Francois et al.Tissue transglutaminase 2 regulates tumor cell tensional homeostasis by increasing contractilityJournal of Cell Science2020ISSN 1477-9137
Papalazarou, Vassilis et al.The creatine–phosphagen system is mechanoresponsive in pancreatic adenocarcinoma and fuels invasion and metastasisNature Metabolism2020ISSN 2522-5812
Juin, Amelie et al.N-WASP Control of LPAR1 Trafficking Establishes Response to Self-Generated LPA Gradients to Promote Pancreatic Cancer Cell MetastasisDevelopmental Cell2019ISSN 1878-1551
Tedeschi, Andrea et al.ADF/Cofilin-Mediated Actin Turnover Promotes Axon Regeneration in the Adult CNSNeuron2019ISSN 1097-4199
Paonessa, Francesco et al.Microtubules Deform the Nuclear Membrane and Disrupt Nucleocytoplasmic Transport in Tau-Mediated Frontotemporal DementiaCell Reports2019ISSN 2211-1247
Umesalma, Shaikamjad et al.RABL6A inhibits tumor-suppressive PP2A/AKT signaling to drive pancreatic neuroendocrine tumor growthJournal of Clinical Investigation2019ISSN 1558-8238
Stephens, Matthew et al.Mesenteric lymphatic alterations observed during DSS induced intestinal inflammation are driven in a TLR4-PAMP/DAMP discriminative mannerFrontiers in Immunology2019ISSN 1664-3224
Dias Gomes, Martim et al.Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrityNature Communications2019ISSN 2041-1723
Cederquist, Gustav Y. et al.Specification of positional identity in forebrain organoidsNature Biotechnology2019ISSN 1546-1696
Kikuchi, Daniel S. et al.Poldip2 mediates blood-brain barrier disruption in a model of sepsis-associated encephalopathyJournal of Neuroinflammation2019ISSN 1742-2094
Alhasani, Reem Hasaballah et al.Gypenosides protect retinal pigment epithelium cells from oxidative stressFood and Chemical Toxicology2018ISSN 1873-6351
Patnaik, Sarita Rani et al.RPGR protein complex regulates proteasome activity and mediates store-operated calcium entryOncotarget2018ISSN 1949-2553
Zhang, Xiaoqing et al.Alterations of MEK1/2-ERK1/2, IFNγ and Smad2/3 associated Signalling pathways during cryopreservation of ASCs affect their differentiation towards VSMC-like cellsStem Cell Research2018ISSN 1876-7753
Borin, Mirta et al.Rac1 activation links tau hyperphosphorylation and Aß dysmetabolism in Alzheimer's diseaseActa Neuropathologica Communications2018ISSN 2051-5960
Horita, Henrick et al.Utilizing a comprehensive immunoprecipitation enrichment system to identify an endogenous post-translational modification profile for target proteinsJournal of Visualized Experiments2018ISSN 1940-087X
Mangiatordi, Giuseppe Felice et al.Automated identification of structurally heterogeneous and patentable antiproliferative hits as potential tubulin inhibitorsChemical Biology and Drug Design2018ISSN 1747-0285
Meiring, Joyce C.M. et al.Co-polymers of Actin and Tropomyosin Account for a Major Fraction of the Human Actin CytoskeletonCurrent Biology2018ISSN 0960-9822
Cruz-Nova, Pedro et al.The small organic molecule C19 binds and strengthens the KRAS4b-PDEδ complex and inhibits growth of colorectal cancer cells in vitro and in vivo 11 Medical and Health Sciences 1112 Oncology and CarcinogenesisBMC Cancer2018ISSN 1471-2407
Casique-Aguirre, Diana et al.KRas4B-PDE6δ complex stabilization by small molecules obtained by virtual screening affects Ras signaling in pancreatic cancer 06 Biological Sciences 0601 Biochemistry and Cell BiologyBMC Cancer2018ISSN 1471-2407
McQueeney, Kelley E. et al.Targeting ovarian cancer and endothelium with an allosteric PTP4A3 phosphatase inhibitorOncotarget2018ISSN 1949-2553
Zhu, Yili et al.An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated ProteinBio-Protocol2018ISSN 2331--8325
Proto, Jonathan D. et al.Regulatory T Cells Promote Macrophage Efferocytosis during Inflammation ResolutionImmunity2018ISSN 1097-4180
Albalawi, Aishah et al.Protective effect of carnosic acid against acrylamide-induced toxicity in RPE cellsFood and Chemical Toxicology2017ISSN 1873-6351
Mangiatordi, Giuseppe Felice et al.Novel chemotypes targeting tubulin at the colchicine binding site and unbiasing P-glycoproteinEuropean Journal of Medicinal Chemistry2017ISSN 1768-3254
Huang, Jia Lin et al.Lipoprotein-biomimetic nanostructure enables efficient targeting delivery of siRNA to Ras-activated glioblastoma cells via macropinocytosisNature Communications2017ISSN 2041-1723
Hendricks, Louie et al.Milk fat globule-epidermal growth factor-factor VIII–derived peptide MSP68 is a cytoskeletal immunomodulator of neutrophils that inhibits Rac1Journal of Surgical Research2017ISSN 1095-8673
Lampi, Marsha C. et al.Simvastatin ameliorates matrix stiffness-mediated endothelial monolayer disruptionPLoS ONE2016ISSN 1932-6203
Chen, Weihua et al.High RhoA expression at the tumor front in clinically localized prostate cancer and association with poor tumor differentiationOncology Letters2016ISSN 1792-1082
Zan, G. Y. et al.p38 mitogen-activated protein kinase activation in amygdala mediates κ opioid receptor agonist U50,488H-induced conditioned place aversionNeuroscience2016ISSN 1873-7544
Chen, Lihua et al.Smooth muscle-alpha actin inhibits vascular smooth muscle cell proliferation and migration by inhibiting rac1 activityPLoS ONE2016ISSN 1932-6203
Castillo-Pichardo, Linette et al.Dietary grape polyphenol resveratrol increases mammary tumor growth and metastasis in immunocompromised mice2013PMID 23298290
Takefuji, Mikito et al.RhoGEF12 controls cardiac remodeling by integrating G protein- and integrindependent signaling cascadesJournal of Experimental Medicine2013ISSN 0022-1007
Gil-Henn, H. et al.Arg/Abl2 promotes invasion and attenuates proliferation of breast cancer in vivoOncogene2013ISSN 0950-9232
Zilberter, Misha et al.Dietary energy substrates reverse early neuronal hyperactivity in a mouse model of Alzheimer's diseaseJournal of Neurochemistry2013ISSN 1471--4159
Padró, Teresa et al.LDL-induced impairment of human vascular smooth muscle cells repair function is reversed by HMG-CoA reductase inhibitionPLoS ONE2012ISSN 1932-6203
Liu, Yao et al.Actin polymerization-dependent increase in synaptic Arc/Arg3.1 expression in the Amygdala is crucial for the expression of aversive memory associated with drug withdrawalJournal of Neuroscience2012ISSN 0270-6474
Ray, Anasuya et al.Hedgehog signaling pathway regulates the growth of ovarian cancer spheroid forming cellsInternational Journal of Oncology2011ISSN 1019-6439
Hook, Jeff et al.Functional identity of the gamma tropomyosin gene: Implications for embryonic development, reproduction and cell viabilityBioArchitecture2011ISSN 1949-100X
Aguilar, Hector N. et al.Quantification of rapid myosin regulatory light chain phosphorylation using high-throughput in-cell western assays: Comparison to western immunoblotsPLoS ONE2010ISSN 1932-6203
Romero, Ana M. et al.Chronic ethanol exposure alters the levels, assembly, and cellular organization of the actin cytoskeleton and microtubules in hippocampal neurons in primary cultureToxicological Sciences2010ISSN 1096-6080

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