Anti-Cdc42: Mouse Mab

Anti-Cdc42: Mouse Mab


Cdc42 (mol. wt. 21 kDa) belongs to the Rho-family of small G-proteins. The Rho family consists of at least 22 members, the most extensively characterized of which are the Rac1, RhoA and Cdc42 proteins. In common with all other small G-proteins, Rho family proteins act as molecular switches that transmit cellular signals through an array of effector proteins. The family mediates a diverse number of cellular responses including cytoskeletal reorganization, regulation of transcription, apoptosis and neuronal morphology.

Proteins within the Rho-family share 40-95% amino acid identity within their GTPase domains.


> ACD04 has superior lot-to-lot consistency: all Cytoskeleton Inc. antibodies are QC'd for highly reproducible signal and detection.

> ACD04 is priced favorably compared to other Cdc42 antibodies (see table below) 

The anti-Cdc42 antibody is a mouse monoclonal antibody. The antibody was raised against a peptide sequence of human Cdc42 (amino acids 150-182), it specifically recognizes Cdc42 in a wide range of species, including mammalian, reptile and avian and amphibian sources. Quality Control analysis has shown that the antibody does not recognize Rac1. ACD04 is supplied as a lyophilized white powder. Each Lot of antibody is quality controlled to provide a high batch to batch consistency.



Figure 1: Western Blot: Demonstration of ACD04 sensitivity & specificity

Cell extracts were separated by SDS-PAGE and transferred to a PVDF membrane according to the method given in the associated datasheet. Anti-Cdc42 was diluted 1 in 1000 in TBST and western analysis was performed as detailed in the Western Blot Method section. The following lysates were run on the gel: Lane 1: 20 μg A431 cells, Lane 2: 20 μg HeLa cells, Lane 3: 20 μg porcine brain, Lane 4: 500 μg His-tagged Rac1 protein (m.wt. 21 kD). The native Cdc42 band is visible at approximately 23 kD. Exposure time 2 minutes.

For product Datasheets and MSDSs please click on the PDF links below.

Sample Size Datasheet (Cat. ACD04-S):  

If you have any questions concerning this product, please contact our Technical Service department at

Select list of citations of our previous Cdc42 antibody (ACD03).  These references may be a useful tool, as ACD04 and ACD03 performed very similarly in our various QC applications. 

AuthorTitleJournalYearArticle Link
Jayabal, Panneerselvam et al.Roles of USP1 in Ewing sarcomaGenes & Cancer2024PMID 38323120
Ledoux, Benjamin et al.Plasma membrane nanodeformations promote actin polymerization through CIP4/CDC42 recruitment and regulate type II IFN signalingScience Advances2023ISSN 2375-2548
Fu, Lisheng et al.Septin11 promotes hepatocellular carcinoma cell motility by activating RhoA to regulate cytoskeleton and cell adhesionCell Death & Disease 2023 14:42023ISSN 2041--4889
Serwe, Guillaume et al.CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signallingNature Communications 2023 14:12023ISSN 2041--1723
Kamata, Tamihiro et al.Statins mediate anti- and pro-tumourigenic functions by remodelling the tumour microenvironmentDMM Disease Models and Mechanisms2022ISSN 1754-8411
Flores-Muñoz, Carolina et al.The Long-Term Pannexin 1 Ablation Produces Structural and Functional Modifications in Hippocampal NeuronsCells2022ISSN 2073-4409
Chen, Bo et al.Transient neuroinflammation following surgery contributes to long-lasting cognitive decline in elderly rats via dysfunction of synaptic NMDA receptorJournal of Neuroinflammation2022ISSN 1742-2094
Tyckaert, François et al.Rac1, the actin cytoskeleton and microtubules are key players in clathrin-independent endophilin-A3-mediated endocytosisJournal of cell science2022ISSN 1477-9137
Kamata, Tamihiro et al.Statins mediate anti- and pro-tumourigenic functions by remodelling the tumour microenvironmentDMM Disease Models and Mechanisms2022ISSN 1754-8411
Li, Jingxuan et al.Eva1a ameliorates atherosclerosis by promoting re-endothelialization of injured arteries via Rac1/Cdc42/Arpc1bCardiovascular Research2021ISSN 1755-3245
Barbera, Stefano et al.The C-type lectin CD93 controls endothelial cell migration via activation of the Rho family of small GTPasesMatrix Biology2021ISSN 1569-1802
Jayabal, Panneerselvam et al.NELL2-cdc42 signaling regulates BAF complexes and Ewing sarcoma cell growthCell Reports2021ISSN 2211-1247
Howden, Jake D. et al.α2β1 integrins spatially restrict Cdc42 activity to stabilise adherens junctionsBMC biology2021ISSN 1741-7007
Hosseini, Kamran et al.EMT-Induced Cell-Mechanical Changes Enhance Mitotic Rounding StrengthAdvanced Science2020ISSN 2198-3844
Gorisse, Laetitia et al.Ubiquitination of the scaffold protein IQGAP1 diminishes its interaction with and activation of the Rho GTPase CDC42Journal of Biological Chemistry2020ISSN 1083-351X
Oni, Tobiloba E. et al.SOAT1 promotes mevalonate pathway dependency in pancreatic cancerJournal of Experimental Medicine2020ISSN 1540-9538
Gu, Jiawen et al.Rho-GEF trio regulates osteoclast differentiation and function by Rac1/Cdc42Experimental Cell Research2020ISSN 1090-2422
Singh, Rajesh K. et al.Dynamic Actin Reorganization and Vav/Cdc42-Dependent Actin Polymerization Promote Macrophage Aggregated LDL (Low-Density Lipoprotein) Uptake and CatabolismArteriosclerosis, Thrombosis, and Vascular Biology2019ISSN 1524-4636
Huang, Yuxing et al.Arp2/3-branched actin maintains an active pool of GTP-RhoA and controls RhoA abundanceCells2019ISSN 2073-4409
Dupraz, Sebastian et al.RhoA Controls Axon Extension Independent of Specification in the Developing BrainCurrent Biology2019ISSN 0960-9822
Vestre, Katharina et al.Rab6 regulates cell migration and invasion by recruiting Cdc42 and modulating its activityCellular and Molecular Life Sciences2019ISSN 1420-9071
MacKeil, Jodi L. et al.Phosphodiesterase 3B (PDE3B) antagonizes the anti-angiogenic actions of PKA in human and murine endothelial cellsCellular Signalling2019ISSN 1873-3913
Dudvarski Stanković, Nevenka et al. EGFL7 enhances surface expression of integrin α 5 β 1 to promote angiogenesis in malignant brain tumors EMBO Molecular Medicine2018ISSN 1757--4676
Wu, Nan et al.RCC2 over-expression in tumor cells alters apoptosis and drug sensitivity by regulating Rac1 activationBMC Cancer2018ISSN 1471-2407
Peretti, Amanda S. et al.The R-Enantiomer of Ketorolac Delays Mammary Tumor Development in Mouse Mammary Tumor Virus-Polyoma Middle T Antigen (MMTV-PyMT) MiceAmerican Journal of Pathology2018ISSN 1525-2191
Liu, Xiaolei et al.Rasip1 controls lymphatic vessel lumen maintenance by regulating endothelial cell junctionsDevelopment (Cambridge)2018ISSN 1477-9129
Lam, Jonathan G.T. et al.Host cell perforation by listeriolysin O (LLO) activates a Ca2+-dependent cPKC/Rac1/Arp2/3 signaling pathway that promotes Listeria monocytogenes internalization independently of membrane resealingMolecular Biology of the Cell2018ISSN 1939-4586
Li, Cao et al.Regulation of staphylococcus aureus infection of macrophages by CD44, reactive oxygen species, and acid sphingomyelinaseAntioxidants and Redox Signaling2018ISSN 1557-7716
Ghézali, Grégory et al.Connexin 30 controls astroglial polarization during postnatal brain developmentDevelopment (Cambridge)2018ISSN 1477-9129
Kawasaki, Natsumi et al.TUFT1 interacts with RABGAP1 and regulates mTORC1 signalingCell Discovery2018ISSN 2056-5968
Stypulkowski, Ewa et al.The depalmitoylase APT1 directs the asymmetric partitioning of Notch and Wnt signaling during cell divisionScience Signaling2018ISSN 1937-9145
Vodicska, Barbara et al.MISP regulates the IQGAP1/Cdc42 complex to collectively orchestrate spindle orientation and mitotic progressionScientific Reports2018ISSN 2045-2322
Bucka, Kenneth B. et al.Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturationMolecular Biology of the Cell2017ISSN 1939-4586
Chang, Ting Ya et al.Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machineryeLife2017ISSN 2050-084X
Saito, Masaki et al.Tctex‐1 controls ciliary resorption by regulating branched actin polymerization and endocytosisEMBO reports2017ISSN 1469--221X
Takeuchi, Hiroki et al.Intracellular periodontal pathogen exploits recycling pathway to exit from infected cellsCellular Microbiology2016ISSN 1462-5822
Seidelin, Jakob Benedict et al.Cellular inhibitor of apoptosis protein 2 controls human colonic epithelial restitution, migration, and Rac1 activationAmerican Journal of Physiology - Gastrointestinal and Liver Physiology2015ISSN 1522-1547
Thomas, Audrey et al.Involvement of the Rac1-IRSp53-Wave2-Arp2/3 Signaling Pathway in HIV-1 Gag Particle Release in CD4 T CellsJournal of Virology2015ISSN 0022--538X
Liu, Chunqiao et al.Null and hypomorph Prickle1 alleles in mice phenocopy human Robinow syndrome and disrupt signaling downstream of Wnt5aBiology Open2014ISSN 2046-6390
David, Muriel D. et al.The RhoGAP ARHGAP19 controls cytokinesis and chromosome segregation in T lymphocytesJournal of Cell Science2014ISSN 0021-9533
Valtcheva, Nadejda et al.The orphan adhesion G protein-coupled receptor GPR97 regulates migration of lymphatic endothelial cells via the small GTPases RhoA and Cdc42Journal of Biological Chemistry2013ISSN 0021-9258
Barrio, Laura et al.TLR4 Signaling Shapes B Cell Dynamics via MyD88-Dependent Pathways and Rac GTPasesThe Journal of Immunology2013ISSN 0022--1767
Steele, Brian M. et al.WNT-3a modulates platelet function by regulating small GTPase activityFEBS Letters2012ISSN 0014-5793
Mercer, Jason et al.Vaccinia virus strains use distinct forms of macropinocytosis for host-cell entryProceedings of the National Academy of Sciences of the United States of America2010ISSN 0027-8424


Question 1: What is the antigen that this antibody was raised against?

Answer 1: The antibody has been raised against a peptide sequence of human Cdc42 (amino acids 150-182).


Question 2:  What species has this antibody been tested against?

Answer 2:  This antibody is recommended for western blot detection of Cdc42 in human, mouse, rat and bovine cell/tissue extracts.



 If you have any questions concerning this product, please contact our Technical Service department at