Anti-Cdc42: Mouse Mab

Anti-Cdc42: Mouse Mab


Cdc42 (mol. wt. 21 kDa) belongs to the Rho-family of small G-proteins. The Rho family consists of at least 22 members, the most extensively characterized of which are the Rac1, RhoA and Cdc42 proteins. In common with all other small G-proteins, Rho family proteins act as molecular switches that transmit cellular signals through an array of effector proteins. The family mediates a diverse number of cellular responses including cytoskeletal reorganization, regulation of transcription, apoptosis and neuronal morphology.

Proteins within the Rho-family share 40-95% amino acid identity within their GTPase domains.


> ACD04 has superior lot-to-lot consistency: all Cytoskeleton Inc. antibodies are QC'd for highly reproducible signal and detection.

> ACD04 is priced favorably compared to other Cdc42 antibodies (see table below) 

The anti-Cdc42 antibody is a mouse monoclonal antibody. The antibody was raised against a peptide sequence of human Cdc42 (amino acids 150-182), it specifically recognizes Cdc42 in a wide range of species, including mammalian, reptile and avian and amphibian sources. Quality Control analysis has shown that the antibody does not recognize Rac1. ACD04 is supplied as a lyophilized white powder. Each Lot of antibody is quality controlled to provide a high batch to batch consistency.



Figure 1: Western Blot: Demonstration of ACD04 sensitivity & specificity

Cell extracts were separated by SDS-PAGE and transferred to a PVDF membrane according to the method given in the associated datasheet. Anti-Cdc42 was diluted 1 in 1000 in TBST and western analysis was performed as detailed in the Western Blot Method section. The following lysates were run on the gel: Lane 1: 20 μg A431 cells, Lane 2: 20 μg HeLa cells, Lane 3: 20 μg porcine brain, Lane 4: 500 μg His-tagged Rac1 protein (m.wt. 21 kD). The native Cdc42 band is visible at approximately 23 kD. Exposure time 2 minutes.

For product Datasheets and MSDSs please click on the PDF links below.

Sample Size Datasheet (Cat. ACD04-S):  

If you have any questions concerning this product, please contact our Technical Service department at

Select list of citations of our previous Cdc42 antibody (ACD03).  These references may be a useful tool, as ACD04 and ACD03 performed very similarly in our various QC applications. 

AuthorTitleJournalYearArticle Link
Serwe, Guillaume et al.CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signallingNature Communications 2023 14:12023ISSN 2041--1723
Kamata, Tamihiro et al.Statins mediate anti- and pro-tumourigenic functions by remodelling the tumour microenvironmentDMM Disease Models and Mechanisms2022ISSN 1754-8411
Flores-Muñoz, Carolina et al.The Long-Term Pannexin 1 Ablation Produces Structural and Functional Modifications in Hippocampal NeuronsCells2022ISSN 2073-4409
Chen, Bo et al.Transient neuroinflammation following surgery contributes to long-lasting cognitive decline in elderly rats via dysfunction of synaptic NMDA receptorJournal of Neuroinflammation2022ISSN 1742-2094
Tyckaert, François et al.Rac1, the actin cytoskeleton and microtubules are key players in clathrin-independent endophilin-A3-mediated endocytosisJournal of cell science2022ISSN 1477-9137
Kamata, Tamihiro et al.Statins mediate anti- and pro-tumourigenic functions by remodelling the tumour microenvironmentDMM Disease Models and Mechanisms2022ISSN 1754-8411
Li, Jingxuan et al.Eva1a ameliorates atherosclerosis by promoting re-endothelialization of injured arteries via Rac1/Cdc42/Arpc1bCardiovascular Research2021ISSN 1755-3245
Barbera, Stefano et al.The C-type lectin CD93 controls endothelial cell migration via activation of the Rho family of small GTPasesMatrix Biology2021ISSN 1569-1802
Jayabal, Panneerselvam et al.NELL2-cdc42 signaling regulates BAF complexes and Ewing sarcoma cell growthCell Reports2021ISSN 2211-1247
Howden, Jake D. et al.α2β1 integrins spatially restrict Cdc42 activity to stabilise adherens junctionsBMC biology2021ISSN 1741-7007
Hosseini, Kamran et al.EMT-Induced Cell-Mechanical Changes Enhance Mitotic Rounding StrengthAdvanced Science2020ISSN 2198-3844
Gorisse, Laetitia et al.Ubiquitination of the scaffold protein IQGAP1 diminishes its interaction with and activation of the Rho GTPase CDC42Journal of Biological Chemistry2020ISSN 1083-351X
Oni, Tobiloba E. et al.SOAT1 promotes mevalonate pathway dependency in pancreatic cancerJournal of Experimental Medicine2020ISSN 1540-9538
Gu, Jiawen et al.Rho-GEF trio regulates osteoclast differentiation and function by Rac1/Cdc42Experimental Cell Research2020ISSN 1090-2422
Singh, Rajesh K. et al.Dynamic Actin Reorganization and Vav/Cdc42-Dependent Actin Polymerization Promote Macrophage Aggregated LDL (Low-Density Lipoprotein) Uptake and CatabolismArteriosclerosis, Thrombosis, and Vascular Biology2019ISSN 1524-4636
Huang, Yuxing et al.Arp2/3-branched actin maintains an active pool of GTP-RhoA and controls RhoA abundanceCells2019ISSN 2073-4409
Dupraz, Sebastian et al.RhoA Controls Axon Extension Independent of Specification in the Developing BrainCurrent Biology2019ISSN 0960-9822
Vestre, Katharina et al.Rab6 regulates cell migration and invasion by recruiting Cdc42 and modulating its activityCellular and Molecular Life Sciences2019ISSN 1420-9071
MacKeil, Jodi L. et al.Phosphodiesterase 3B (PDE3B) antagonizes the anti-angiogenic actions of PKA in human and murine endothelial cellsCellular Signalling2019ISSN 1873-3913
Dudvarski Stanković, Nevenka et al. EGFL7 enhances surface expression of integrin α 5 β 1 to promote angiogenesis in malignant brain tumors EMBO Molecular Medicine2018ISSN 1757--4676
Wu, Nan et al.RCC2 over-expression in tumor cells alters apoptosis and drug sensitivity by regulating Rac1 activationBMC Cancer2018ISSN 1471-2407
Peretti, Amanda S. et al.The R-Enantiomer of Ketorolac Delays Mammary Tumor Development in Mouse Mammary Tumor Virus-Polyoma Middle T Antigen (MMTV-PyMT) MiceAmerican Journal of Pathology2018ISSN 1525-2191
Liu, Xiaolei et al.Rasip1 controls lymphatic vessel lumen maintenance by regulating endothelial cell junctionsDevelopment (Cambridge)2018ISSN 1477-9129
Lam, Jonathan G.T. et al.Host cell perforation by listeriolysin O (LLO) activates a Ca2+-dependent cPKC/Rac1/Arp2/3 signaling pathway that promotes Listeria monocytogenes internalization independently of membrane resealingMolecular Biology of the Cell2018ISSN 1939-4586
Li, Cao et al.Regulation of staphylococcus aureus infection of macrophages by CD44, reactive oxygen species, and acid sphingomyelinaseAntioxidants and Redox Signaling2018ISSN 1557-7716
Ghézali, Grégory et al.Connexin 30 controls astroglial polarization during postnatal brain developmentDevelopment (Cambridge)2018ISSN 1477-9129
Kawasaki, Natsumi et al.TUFT1 interacts with RABGAP1 and regulates mTORC1 signalingCell Discovery2018ISSN 2056-5968
Stypulkowski, Ewa et al.The depalmitoylase APT1 directs the asymmetric partitioning of Notch and Wnt signaling during cell divisionScience Signaling2018ISSN 1937-9145
Vodicska, Barbara et al.MISP regulates the IQGAP1/Cdc42 complex to collectively orchestrate spindle orientation and mitotic progressionScientific Reports2018ISSN 2045-2322
Bucka, Kenneth B. et al.Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturationMolecular Biology of the Cell2017ISSN 1939-4586
Chang, Ting Ya et al.Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machineryeLife2017ISSN 2050-084X
Saito, Masaki et al.Tctex‐1 controls ciliary resorption by regulating branched actin polymerization and endocytosisEMBO reports2017ISSN 1469--221X
Takeuchi, Hiroki et al.Intracellular periodontal pathogen exploits recycling pathway to exit from infected cellsCellular Microbiology2016ISSN 1462-5822
Seidelin, Jakob Benedict et al.Cellular inhibitor of apoptosis protein 2 controls human colonic epithelial restitution, migration, and Rac1 activationAmerican Journal of Physiology - Gastrointestinal and Liver Physiology2015ISSN 1522-1547
Thomas, Audrey et al.Involvement of the Rac1-IRSp53-Wave2-Arp2/3 Signaling Pathway in HIV-1 Gag Particle Release in CD4 T CellsJournal of Virology2015ISSN 0022--538X
Liu, Chunqiao et al.Null and hypomorph Prickle1 alleles in mice phenocopy human Robinow syndrome and disrupt signaling downstream of Wnt5aBiology Open2014ISSN 2046-6390
David, Muriel D. et al.The RhoGAP ARHGAP19 controls cytokinesis and chromosome segregation in T lymphocytesJournal of Cell Science2014ISSN 0021-9533
Valtcheva, Nadejda et al.The orphan adhesion G protein-coupled receptor GPR97 regulates migration of lymphatic endothelial cells via the small GTPases RhoA and Cdc42Journal of Biological Chemistry2013ISSN 0021-9258
Barrio, Laura et al.TLR4 Signaling Shapes B Cell Dynamics via MyD88-Dependent Pathways and Rac GTPasesThe Journal of Immunology2013ISSN 0022--1767
Steele, Brian M. et al.WNT-3a modulates platelet function by regulating small GTPase activityFEBS Letters2012ISSN 0014-5793
Mercer, Jason et al.Vaccinia virus strains use distinct forms of macropinocytosis for host-cell entryProceedings of the National Academy of Sciences of the United States of America2010ISSN 0027-8424


Question 1: What is the antigen that this antibody was raised against?

Answer 1: The antibody has been raised against a peptide sequence of human Cdc42 (amino acids 150-182).


Question 2:  What species has this antibody been tested against?

Answer 2:  This antibody is recommended for western blot detection of Cdc42 in human, mouse, rat and bovine cell/tissue extracts.



 If you have any questions concerning this product, please contact our Technical Service department at