Actin protein (>99% pure): bovine cardiac muscle

Actin protein (>99% pure): bovine cardiac muscle
$0.00

Product Uses Include

  • Identification and characterization of cardiac actin binding proteins
  • In vitro cardiac actin polymerization studies

 

Material:
Bovine cardiac muscle actin is supplied as a lyophilized powder. When one vial is reconstituted in 1 ml of Actin Monomer Buffer: 5.0 mM Tris-HCI pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP and 0.5 mM DTT, it is at a final concentration of 1.0 mg/ml.

Purity:
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are > 99% pure.

Biological Activity:
The biological activity is determined in three ways. The first method utilizes pyrene muscle actin as a fluorescent indicator of polymerization (use a 1:10 ratio of AP07 to AD99) . The second involves sedimentation of actin polymer by high speed centrifugation. The third method measures the integrity of the monomer by the specific inhibition of DNase activity. The biological activity is > 90%.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

 

  • For a guide to performing actin polymerizations with this actin product please click here.

AuthorTitleJournalYearArticle Link
Lu, Yi Ju et al.Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunityNature Communications2020ISSN 2041-1723
Seervai, Riyad N.H. et al.The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferaseScience Advances2020ISSN 2375-2548
Sandberg, Alexander et al.Fibrillation and molecular characteristics are coherent with clinical and pathological features of 4-repeat tauopathy caused by MAPT variant G273RNeurobiology of Disease2020ISSN 1095-953X
Mahmud, Zabed et al.Structure and proteolytic susceptibility of the inhibitory C-terminal tail of cardiac troponin IBiochimica et Biophysica Acta (BBA) - General Subjects2019
Yang, Kai Chien et al.Deficiency of nuclear receptor interaction protein leads to cardiomyopathy by disrupting sarcomere structure and mitochondrial respirationJournal of molecular and cellular cardiology2019ISSN 1095--8584
Da’as, Sahar I. et al.Hypertrophic cardiomyopathy-linked variants of cardiac myosin-binding protein C3 display altered molecular properties and actin interactionBiochemical Journal2018ISSN 1470-8728
Manickam, Manoj et al.Exploration of flexible phenylpropylurea scaffold as novel cardiac myosin activators for the treatment of systolic heart failureEuropean Journal of Medicinal Chemistry2017ISSN 1768-3254
Cuellar, Jorge et al.Assisted protein folding at low temperature: Evolutionary adaptation of the antarctic fish chaperonin CCT and its client proteinsBiology Open2014ISSN 2046-6390
Butler, Suzanne C. et al.Inhibitory effects of pectenotoxins from marine algae on the polymerization of various actin isoformsToxicology in Vitro2012ISSN 0887-2333
Orlova, Albina et al.The N-Terminal Domains of Myosin Binding Protein C Can Bind Polymorphically to F-ActinJournal of Molecular Biology2011
Passarelli, Chiara et al.Susceptibility of isolated myofibrils to in vitro glutathionylation: Potential relevance to muscle functionsCytoskeleton2010ISSN 1949--3592
Debold, Edward P. et al.Human actin mutations associated with hypertrophic and dilated cardiomyopathies demonstrate distinct thin filament regulatory properties in vitroJournal of Molecular and Cellular Cardiology2010ISSN 0022--2828

 

Question 1: What is the isotype composition of the cardiac actin from bovine heart muscle?

Answer 1:  Bovine cardiac actin (Cat. # AD99) is composed of 84% a-cardiac and 16% a-skeletal actin isoforms. 

 

Question 2:  How do I measure actin polymerization with the pyrene assay when there is no available pyrene conjugate for cardiac actin? 

Answer 2:  To examine the polymerization of unlabeled cardiac actin (Cat. # AD99), please click here for a polymerization protocol that uses an excess of the unlabeled [cardiac] actin plus 10% of pyrene-labeled skeletal muscle actin.  The pyrene muscle actin will not polymerize on its own at the concentration used in this assay, so the reaction is dependent on unlabeled cardiac actin (Cat. # AD99) for F-actin formation.  In this way, the pyrene-labeled muscle actin is taken up and polymerized to serve as a reporter for polymerization of the unlabeled actin that is present at a much greater concentration.

 

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com