Introduction
Cdc42 is one of most extensively studied member of Rho GTPase family. This family is involved in a wide range of cellular responses, including cytoskeletal reorganization, regulation of transcription, cell migration, cellular transformation and metastasis. Cdc42 is expressed in a large number of different cell types.
Product Uses Include
This antibody is recommended for detection of Cdc42 in human, mouse, rat or other extracts. The following protocols have been tested with this antibody:
| Western Blot | Immunocytochemistry | ELISA | Immunoprecipitation |
| Yes | Yes | No | No |
Material
The anti-Cdc42 antibody (Cat. # ACD03) is a mouse monoclonal antibody that only recognizes Cdc42, not Rac1, Rac2, Rac3, RhoA, RhoB, RhoC or H-Ras. Other commercially available Cdc42 antibodies recognize one or more of these close isoforms Platelet extract (Cat. # EXT01) is included as a positive control. ACD03 is supplied as a lyophilized white powder.
Figure 1: Western blot analysis of purified protein and cell extracts using ACD03 as primary antibody. Recombinant small G-proteins and tissue extracts were separated by SDS-PAGE and transferred to PVDF membrane. ACD03 was diluted to 250 ng/ml (1:1000) in PBST plus 0.1% non-fat milk powder and Western analysis was as described in the Methods section of the datasheet. Lane 1; 25 ng Cdc42-6xHIS, Lane 2; 250 ng RhoA-6xHIS, Lane 3; 250 ng Rac1-6xHIS, Lane 4; 40 µg Swiss 3T3 cell extract, Lane 5; 40 µg bovine brain extract, Lane 6; 40 µg human platelet extract. Note: Cdc42-6xHIS runs at 25kDal whereas the native Cdc42 runs at 22kDal.
Table 1 - Reactivity of other commercially available Cdc42 antibodies
Cdc42 | Rac sub-family | Rho sub-family | Ras sub-family | |
Cytoskeleton, Inc. (Cat. # ACD03) | Yes | No | No | No |
Santa Cruz (Cat. #sc-87-G) | Yes | Yes | Yes | NT |
Cell Signalling (Cat. # 2462) | Yes | Yes | NT | NT |
NT = Not tested
For product Datasheets and MSDSs please click on the PDF links below.
Sample Size Datasheet (Cat. ACD03-S):
Li, J. et al. Eva1a ameliorates atherosclerosis by promoting re-endothelialization of injured arteries via Rac1/Cdc42/Arpc1b. Cardiovasc. Res. (2020) doi:10.1093/cvr/cvaa011.
Gorisse, L. et al. Ubiquitination of the scaffold protein IQGAP1 diminishes its interaction with and activation of the Rho GTPase CDC42. J. Biol. Chem. 295, 4822–4835 (2020).
Huang, Y., Yi, X., Kang, C. & Wu, C. Arp2/3-Branched Actin Maintains an Active Pool of GTP-RhoA and Controls RhoA Abundance. Cells 8, (2019).
Singh, R. K., Haka, A. S., Bhardwaj, P., Zha, X. & Maxfield, F. R. Dynamic Actin Reorganization and Vav/Cdc42-Dependent Actin Polymerization Promote Macrophage Aggregated LDL (Low-Density Lipoprotein) Uptake and Catabolism. Arterioscler. Thromb. Vasc. Biol. 39, 137–149 (2019).
Vestre, K. et al. Rab6 regulates cell migration and invasion by recruiting Cdc42 and modulating its activity. Cell. Mol. Life Sci. 76, 2593–2614 (2019).
MacKeil, J. L. et al. Phosphodiesterase 3B (PDE3B) antagonizes the anti-angiogenic actions of PKA in human and murine endothelial cells. Cell. Signal. 62, (2019).
Singh, R. K., Haka, A. S., Bhardwaj, P., Zha, X. & Maxfield, F. R. Dynamic Actin Reorganization and Vav/Cdc42-Dependent Actin Polymerization Promote Macrophage Aggregated LDL (Low-Density Lipoprotein) Uptake and Catabolism. Arterioscler. Thromb. Vasc. Biol. 39, 137–149 (2019).
Huang, Y., Yi, X., Kang, C. & Wu, C. Arp2/3-Branched Actin Maintains an Active Pool of GTP-RhoA and Controls RhoA Abundance. Cells 8, 1264 (2019).
Dudvarski Stanković, N. et al. EGFL7 enhances surface expression of integrin α 5 β 1 to promote angiogenesis in malignant brain tumors. EMBO Mol. Med. 10, (2018).
Peretti, A. S. et al. The R-Enantiomer of Ketorolac Delays Mammary Tumor Development in Mouse Mammary Tumor Virus-Polyoma Middle T Antigen (MMTV-PyMT) Mice. Am. J. Pathol. 188, 515–524 (2018).
Wu, N. et al. RCC2 over-expression in tumor cells alters apoptosis and drug sensitivity by regulating Rac1 activation. BMC Cancer 18, 1–11 (2018).
Bucka, K. B. et al. Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation. Mol. Biol. Cell 28, 98–110 (2017).
Am. J. Physiol. Gastrointest. Liver Physiol. 2015; 308, G92-G99.
"Cellular inhibitor of apoptosis protein 2 controls human colonic epithelial restitution, migration, and Rac1 activation"
Author(s): Seidelin J.B. et al.
(See PubMed article)
Cell Physiol Biochem. 2015; 36, 1613-1627.
"Neuroglobin Plays a Protective Role in Arsenite-Induced Cytotoxicity by Inhibition of Cdc42 and Rac1GTPases in Rat Cerebellar Granule Neurons"
Author(s): Liu X. et al.
(See PubMed article)
Oncogene. 2014; doi: 10.1038/onc.2014.315.
"LKB1 reduces ROS-mediated cell damage via activation of p38"
Author(s): Xu H.-G. et al.
(See PubMed article)
J. Lipid Res. 2014; 55, 2389-2400.
"Lipid phosphate phosphatase-1 expression in cancer cells attenuates tumor growth and metastasis in mice"
Author(s): Tang X. et al.
(See PubMed article)
Platelets, 2008, Vol. 19, No. 3, Pages 199-210 , DOI: 10.1080/09537100701777303
"Critical roles for the actin cytoskeleton and cdc42 in regulating platelet integrin a2b1."
Author(s): Giordano Pula and Alastair W. Poole.
(See PubMed article)
Journal of Biological Chemistry, 2008; 283, 27230-27238. DOI: 10.1074/jbc.M804520200
"ABL2/ARG Tyrosine Kinase Mediates SEMA3F-induced RhoA Inactivation and Cytoskeleton Collapse in Human Glioma Cells."
Author(s): Akio Shimizu and Akiko Mammoto et al.
(See PubMed article)
Question 1: What is the antigen that this antibody was raised against?
Answer 1: The antibody has been raised against a peptide sequence of human Cdc42 (amino acids 128-138).
Question 2: What species has this antibody been tested against?
Answer 2: This antibody is recommended for western blot detection of Cdc42 in human, mouse, rat and bovine cell/tissue extracts.
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
