Introduction
Cdc42 is one of most extensively studied member of Rho GTPase family. This family is involved in a wide range of cellular responses, including cytoskeletal reorganization, regulation of transcription, cell migration, cellular transformation and metastasis. Cdc42 is expressed in a large number of different cell types.
Product Uses Include
This antibody is recommended for detection of Cdc42 in human, mouse, rat or other extracts. The following protocols have been tested with this antibody:
| Western Blot | Immunocytochemistry | ELISA | Immunoprecipitation |
| Yes | Yes | No | No |
Material
The anti-Cdc42 antibody (Cat. # ACD03) is a mouse monoclonal antibody that only recognizes Cdc42, not Rac1, Rac2, Rac3, RhoA, RhoB, RhoC or H-Ras. Other commercially available Cdc42 antibodies recognize one or more of these close isoforms Platelet extract (Cat. # EXT01) is included as a positive control. ACD03 is supplied as a lyophilized white powder.
Figure 1: Western blot analysis of purified protein and cell extracts using ACD03 as primary antibody. Recombinant small G-proteins and tissue extracts were separated by SDS-PAGE and transferred to PVDF membrane. ACD03 was diluted to 250 ng/ml (1:1000) in PBST plus 0.1% non-fat milk powder and Western analysis was as described in the Methods section of the datasheet. Lane 1; 25 ng Cdc42-6xHIS, Lane 2; 250 ng RhoA-6xHIS, Lane 3; 250 ng Rac1-6xHIS, Lane 4; 40 µg Swiss 3T3 cell extract, Lane 5; 40 µg bovine brain extract, Lane 6; 40 µg human platelet extract. Note: Cdc42-6xHIS runs at 25kDal whereas the native Cdc42 runs at 22kDal.
Table 1 - Reactivity of other commercially available Cdc42 antibodies
Cdc42 | Rac sub-family | Rho sub-family | Ras sub-family | |
Cytoskeleton, Inc. (Cat. # ACD03) | Yes | No | No | No |
Santa Cruz (Cat. #sc-87-G) | Yes | Yes | Yes | NT |
Cell Signalling (Cat. # 2462) | Yes | Yes | NT | NT |
NT = Not tested
For product Datasheets and MSDSs please click on the PDF links below.
Sample Size Datasheet (Cat. ACD03-S):
Am. J. Physiol. Gastrointest. Liver Physiol. 2015; 308, G92-G99.
"Cellular inhibitor of apoptosis protein 2 controls human colonic epithelial restitution, migration, and Rac1 activation"
Author(s): Seidelin J.B. et al.
(See PubMed article)
Cell Physiol Biochem. 2015; 36, 1613-1627.
"Neuroglobin Plays a Protective Role in Arsenite-Induced Cytotoxicity by Inhibition of Cdc42 and Rac1GTPases in Rat Cerebellar Granule Neurons"
Author(s): Liu X. et al.
(See PubMed article)
Oncogene. 2014; doi: 10.1038/onc.2014.315.
"LKB1 reduces ROS-mediated cell damage via activation of p38"
Author(s): Xu H.-G. et al.
(See PubMed article)
J. Lipid Res. 2014; 55, 2389-2400.
"Lipid phosphate phosphatase-1 expression in cancer cells attenuates tumor growth and metastasis in mice"
Author(s): Tang X. et al.
(See PubMed article)
Platelets, 2008, Vol. 19, No. 3, Pages 199-210 , DOI: 10.1080/09537100701777303
"Critical roles for the actin cytoskeleton and cdc42 in regulating platelet integrin a2b1."
Author(s): Giordano Pula and Alastair W. Poole.
(See PubMed article)
Journal of Biological Chemistry, 2008; 283, 27230-27238. DOI: 10.1074/jbc.M804520200
"ABL2/ARG Tyrosine Kinase Mediates SEMA3F-induced RhoA Inactivation and Cytoskeleton Collapse in Human Glioma Cells."
Author(s): Akio Shimizu and Akiko Mammoto et al.
(See PubMed article)
Question 1: What is the antigen that this antibody was raised against?
Answer 1: The antibody has been raised against a peptide sequence of human Cdc42 (amino acids 128-138).
Question 2: What species has this antibody been tested against?
Answer 2: This antibody is recommended for western blot detection of Cdc42 in human, mouse, rat and bovine cell/tissue extracts.
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
