Live Cell Imaging Reagents

Cytoskeleton Inc. - Fluorescent Proteins and Reagents

Cytoskeleton offers several reagents for live-cell research including fluorescent actin, ECM, and tubulin proteins, as well as, cell permeable protein activators and inhibitors. This includes our new HiLyte™488 actin and HiLyte™555 actin tools that provide more versatility for researchers while complementing our existing labeled actin and tubulin tools that are used in an array of applications.

Spirochrome Probes For Bioimaging

The SiR- , SPY-, PK-, and Flipper-TR Live-cell imaging probes developed by Spirochrome are cell-permeable compounds which label microtubules, F-actin , Lysosomes, membrane, mitochondria, and chromosomal DNA in living cells. SPY™ probes improve upon the SiR live cell imaging technology while also expanding the fluorophore labeling options for the study of F-actin, microtubules, and DNA in living cells.

MemGlow™ Probes For Bioimaging: From The MemBright Family Of Probes

Cytoskeleton Inc. offers an array of innovative probes to visualize the cellular plasma membrane, which was originally developed by the makers of the MemBright™ probes. MemGlow™ probes provide high photostability, exhibit excellent fluorescent quantum yields, and some of them are super-resolution compatible. Also available are NR Polarity Probes which are solvatochromic photostable plasma membrane-targeting dyes.

HiLyte™555 labeled actin

See More Fluorescent Actins

SiR Actin Labeled Cells

See More Spirochrome Probes

NR12A Membrane Probe

See More MemGlow Probes

Cytoskeleton's live cell imaging products have been cited hundreds of times over the past two decades. A select few are described here. More individual product citations listed on each product page.

Author	Title	Journal	Year	Article Link
Zhou, Qing et al.	An orphan kinesin in Trypanosoma brucei regulates hook complex assembly and Golgi biogenesis	mBio	2024	ISSN 2150--7511
Sun, Shuangshuang et al.	Metabolic regulation of cytoskeleton functions by HDAC6-catalyzed α-tubulin lactylation	Nature Communications 2024 15:1	2024	ISSN 2041--1723
Salvador-Garcia, David et al.	A force-sensitive mutation reveals a non-canonical role for dynein in anaphase progression	The Journal of cell biology	2024	ISSN 1540-8140
Singh, Kashish et al.	Molecular mechanism of dynein-dynactin complex assembly by LIS1	Science	2024	ISSN 1095-9203
Rai, Dipti et al.	CAMSAPs and nucleation-promoting factors control microtubule release from γ-TuRC	Nature Cell Biology	2024	ISSN 1476--4679
Polley, Soumitra et al.	Stable kinetochore‐microtubule attachment requires loop‐dependent Ndc80‐Ndc80 binding	The EMBO Journal	2023	ISSN 0261--4189
Nishida, Kohei et al.	Effects of three microtubule-associated proteins (MAP2, MAP4, and Tau) on microtubules’ physical properties and neurite morphology	Scientific Reports	2023	ISSN 2045--2322
De Abreu, Isadora Rocha et al.	A molecular analysis of substituted phenylethylamines as potential microtubule targeting agents through in silico methods and in vitro microtubule-polymerization activity	Scientific Reports	2023	ISSN 2045--2322
Singh, Rajendra K. et al.	Coordinated Biophysical Stimulation of MSCs via Electromagnetized Au-Nanofiber Matrix Regulates Cytoskeletal-to-Nuclear Mechanoresponses and Lineage Specification	Advanced Functional Materials	2023	ISSN 1616--3028
Castrogiovanni, Cédric et al.	Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubules	Nature Communications	2022	ISSN 2041--1723
Masucci, Erin M. et al.	Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites	Molecular Biology of the Cell	2022	ISSN 1939-4586
Henty-Ridilla, Jessica L.	Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy	JoVE (Journal of Visualized Experiments)	2022	ISSN 1940--087X
Sasanpour, Mehrzad et al.	Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics	JoVE (Journal of Visualized Experiments)	2022	ISSN 1940--087X
Baron, Desiree M et al.	ALS-associated KIF5A mutations abolish autoinhibition resulting in a toxic gain of function.	Cell reports	2022	ISSN 2211--1247
Cheng, Xianrui et al.	Xenopus laevis egg extract preparation and live imaging methods for visualizing dynamic cytoplasmic organization	Journal of Visualized Experiments	2021	ISSN 1940-087X
Goldstein-Levitin, Alina et al.	Intracellular functions and motile properties of bi-directional kinesin-5 cin8 are regulated by neck linker docking	eLife	2021	ISSN 2050-084X
Lee, Gloria et al.	Active cytoskeletal composites display emergent tunable contractility and restructuring	Soft Matter	2021	ISSN 1744-6848
Aher, Amol et al.	CLASP Mediates Microtubule Repair by Restricting Lattice Damage and Regulating Tubulin Incorporation	Current Biology	2020	ISSN 1879-0445
Nolet, Felix E. et al.	Nuclei determine the spatial origin of mitotic waves	eLife	2020	ISSN 2050-084X
Chen, Keyu et al.	Giant ankyrin-B suppresses stochastic collateral axon branching through direct interaction with microtubules	Journal of Cell Biology	2020	ISSN 1540-8140
Rodríguez-García, Ruddi et al.	Mechanisms of Motor-Independent Membrane Remodeling Driven by Dynamic Microtubules	Current Biology	2020	ISSN 1879-0445
Farhadi, Leila et al.	Actin and microtubule crosslinkers tune mobility and control co-localization in a composite cytoskeletal network	Soft Matter	2020	ISSN 1744-6848
Nakos, Konstantinos et al.	Regulation of microtubule plus end dynamics by septin 9	Cytoskeleton	2019	ISSN 1949-3592
Guedes-Dias, Pedro et al.	Kinesin-3 Responds to Local Microtubule Dynamics to Target Synaptic Cargo Delivery to the Presynapse	Current Biology	2019	ISSN 0960-9822
Nakos, Konstantinos et al.	Septin 2/6/7 complexes tune microtubule plus-end growth and EB1 binding in a concentration- And filament-dependent manner	Molecular Biology of the Cell	2019	ISSN 1939-4586
Fan, Yuanwei et al.	The Arabidopsis SPIRAL2 Protein Targets and Stabilizes Microtubule Minus Ends	Current Biology	2018	ISSN 0960-9822
Colin, Alexandra et al.	Actin-Network Architecture Regulates Microtubule Dynamics	Current Biology	2018	ISSN 0960-9822
McClintock, Mark A. et al.	RNA-directed activation of cytoplasmic dynein-1 in reconstituted transport RNPs	eLife	2018	ISSN 2050-084X
Aher, Amol et al.	CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain	Developmental Cell	2018	ISSN 1878-1551
Melo, Esther et al.	HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model	EBioMedicine	2018	ISSN 2352-3964
Zhu, Yili et al.	An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein	Bio-Protocol	2018	ISSN 2331--8325
Zhang, Rui et al.	Interplay of structure, elasticity, and dynamics in actin-based nematic materials	Proceedings of the National Academy of Sciences of the United States of America	2017	ISSN 1091-6490
Kandel, Mikhail E. et al.	Label-Free Imaging of Single Microtubule Dynamics Using Spatial Light Interference Microscopy	ACS Nano	2017	ISSN 1936-086X
Arellano-Santoyo, Hugo et al.	A Tubulin Binding Switch Underlies Kip3/Kinesin-8 Depolymerase Activity	Developmental Cell	2017	ISSN 1878-1551
Britto, Mishan et al.	Schizosaccharomyces pombe kinesin-5 switches direction using a steric blocking mechanism	Proceedings of the National Academy of Sciences of the United States of America	2016	ISSN 1091-6490
Taberner, Núria et al.	Reconstituting Functional Microtubule-Barrier Interactions	Methods in Cell Biology	2014	ISSN 0091-679X
DeBerg, Hannah A. et al.	Motor domain phosphorylation modulates kinesin-1 transport	Journal of Biological Chemistry	2013	ISSN 0021-9258
Rostovtseva, Tatiana K. et al.	Membrane Lipid Composition Regulates Tubulin Interaction with Mitochondrial Voltage-dependent Anion Channel *	Journal of Biological Chemistry	2012	ISSN 0021--9258
Hara, Masatoshi et al.	Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factor	Nature Communications	2012	ISSN 2041-1723