Alpha Tubulin N-Acetyltransferase 1 (2-236) protein (Human recombinant)
Alpha Tubulin N-Acetyltransferase 1 (2-236) protein (Human recombinant)
Product Uses Include
- In Vitro Acetylation of Tubulin
- Acetylation Studies with Tubulin
- In Vitro Acetylation of other known targets
Material
The human αTAT1 protein (2-236) was produced in a bacterial expression system. The recombinant protein is untagged and has an approximate molecular weight of 27 kDa. The protein is lyophilized and supplied as a white solid.
Purity
A 20 μg sample of αTAT1 purity is determined by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gradient gel. Acetylated tubulin protein was determined to be 90% pure. (see Figure 1)
Figure 1: Purity analysis of αTAT1 residues 2-236 Protein quantitation was performed using the Precision Red Protein Assay Reagent (Cat. # ADV02). Molecular weight markers are from Invitrogen (Mark 12).
Biological Activity
The biological activity of αTAT1 is assessed by its ability to acetylate tubulin during tubulin polymerization by measuring tubulin acetylation via Western analysis (Figure. 2). The results show an increase in K40-Acetylated a-tubulin with the addition of αTAT1.
Figure 2. Tubulin acetylation by aTAT1
Each reaction contains 40 mM of tubulin (Cat. # T240) and decreasing reaction ratios of 1:12, 1:24, 1:48, and 1:120 Tubulin:αTAT1 proteins. Tubulin was polymerized at 5 mg/ml with or without αTAT1 for 60 minutes at 30oC.
References
- Barra H.S., et al. (1974) Biochem. Biophys. Res. Commun.60:1384-1390.
- Eshun-Wilson L., et al. (2019) PNAS 116:10366-10371.
- Dompierre et al. (2007) J . Neurosci. 27:3571-3583.
- Reed et al. (2006) Curr. Biol. 16:2166-2172.
- Nekooki-Machida and Hagiwara. (2020) Med Mol Morphol. 53:191–197.
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If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com.
