Product Uses Include
- Tracking cell movement through a 3D matrix
- Tracking cell movement across a glass slide
- Observation of fibronectin matrix assembly and cell adhesion.
- FACS analysis of fibronectin binding cells
Material
Fibronectin purified from bovine plasma and has been modified to contain a covalently linked rhodamine fluorescent dye. An activated ester of rhodamine has been used to label the protein with a labeling stoichiometry of 0.6 dyes per protein molecule. No free dye is apparent in the final product. Fibronectin has an approximate molecular weight of 250 kDa. FNR01 (20 µg of protein) is provided as a lyophilized powder.
Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are >90% pure. No free dye is apparent in the final product.
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Figure 1: Rhodamine Fibronectin Purity Determination. A 20 µg sample of rhodamine fibronectin (molecular weight approx. 250 kDa) was separated by electrophoresis oin a 4-20% SDS-PAGE system and stained with Coomassie Blue.
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For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com
Product Description
MSDS
Datasheet
Cat #
Nagase K, Watanabe M, Kikuchi A, Yamato M, Okano T. (2010). Thermo-Responsive Polymer Brushes as Intelligent Biointerfaces: Preparation via ATRP and Characterization.. Macromol Biosci.
Robinson, E. E., Foty, R. A. and Corbett, S. A. (2004). Fibronectin matrix assembly regulates α5β1-mediated cell cohesion. Mol. Biol. Cell 15, 973-981.
Brock, A., Chang, E., Ho, C. C., LeDuc, P., Jiang, X., Whitesides, G. M. and Ingber, D. E. (2003). Geometric determinants of directional cell motility revealed using microcontact printing. Langmuir 19, 1611-1617.
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